HIGH-SENSITIVITY ANALYSIS OF SIALYL-OLIGOSACCHARIDE GLYCOSYLATION SITES IN GLYCOPROTEINS BY MINIATURIZED TRYPTIC DIGESTION AND MICROCOLUMN LIQUID-CHROMATOGRAPHY

被引：10

作者：

SHIROTA, O ^{[1
]}

RICE, D ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 205卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90422-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive analytical procedure was developed to assess the site of glycosylation in a model glycoprotein, bovine fetuin. First, sample cleavage with immobilized trypsin and the peptide map development are accomplished by microcolumn liquid chromatography. Second, the sialic acids content is measured fluorometrically using their precolumn conversion to quinoxaline derivatives. A unique preconcentration system was developed to secure sensitivity of the second measurement. © 1992.

引用

页码：189 / 192

页数：4

共 15 条

[1] STRUCTURAL CLASSIFICATION OF CARBOHYDRATES IN GLYCOPROTEINS BY MASS-SPECTROMETRY AND HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY
BARR, JR
ANUMULA, KR
VETTESE, MB
TAYLOR, PB
CARR, SA
[J]. ANALYTICAL BIOCHEMISTRY, 1991, 192 (01) : 181 - 192
[2] ASSIGNMENT OF STRUCTURES TO OLIGOSACCHARIDES PRODUCED BY ENZYMATIC DEGRADATION OF A BETA-D-GLUCAN FROM BARLEY BY H-1-NMR AND C-13-NMR SPECTROSCOPY
BOCK, K
DUUS, JO
NORMAN, B
PEDERSEN, S
[J]. CARBOHYDRATE RESEARCH, 1991, 211 (02) : 219 - 233
[3] QUANTITATIVE ANALYTICAL ASPECTS OF REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH SLURRY-PACKED CAPILLARY COLUMNS
BORRA, C
HAN, SM
NOVOTNY, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 385 : 75 - 85
[4] SELECTIVE DETERMINATION OF ARGININE-CONTAINING AND TYROSINE-CONTAINING PEPTIDES USING CAPILLARY ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE DETECTION
COBB, KA
NOVOTNY, MV
[J]. ANALYTICAL BIOCHEMISTRY, 1992, 200 (01) : 149 - 155
[5] HIGH-SENSITIVITY PEPTIDE-MAPPING BY CAPILLARY ZONE ELECTROPHORESIS AND MICROCOLUMN LIQUID-CHROMATOGRAPHY, USING IMMOBILIZED TRYPSIN FOR PROTEIN DIGESTION
COBB, KA
NOVOTNY, M
[J]. ANALYTICAL CHEMISTRY, 1989, 61 (20) : 2226 - 2231
[6] STRUCTURES OF ASPARAGINE-LINKED OLIGOSACCHARIDES OF THE GLYCOPROTEIN FETUIN HAVING SIALIC-ACID LINKED TO N-ACETYLGLUCOSAMINE
CUMMING, DA
HELLERQVIST, CG
HARRISBRANDTS, M
MICHNICK, SW
CARVER, JP
BENDIAK, B
[J]. BIOCHEMISTRY, 1989, 28 (15) : 6500 - 6512
[7] DZIEGIELEWSKA KM, 1990, J BIOL CHEM, V265, P4354
[8] PERFORMANCE EVALUATION OF SLURRY-PACKED CAPILLARY COLUMNS FOR LIQUID-CHROMATOGRAPHY
GLUCKMAN, JC
HIROSE, A
MCGUFFIN, VL
NOVOTNY, M
[J]. CHROMATOGRAPHIA, 1983, 17 (06) : 303 - 309
[9] HIGHLY SENSITIVE DETERMINATION OF N-ACETYL-NEURAMINIC AND N-GLYCOLYLNEURAMINIC ACIDS IN HUMAN-SERUM AND URINE AND RAT SERUM BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
HARA, S
YAMAGUCHI, M
TAKEMORI, Y
NAKAMURA, M
OHKURA, Y
[J]. JOURNAL OF CHROMATOGRAPHY, 1986, 377 : 111 - 119
[10] KELLERMANN J, 1989, J BIOL CHEM, V264, P14121

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