THE INFLUENCE OF PEPTIDE STRUCTURE ON TRANSPORT ACROSS CACO-2 CELLS

被引:196
作者
CONRADI, RA [1 ]
HILGERS, AR [1 ]
HO, NFH [1 ]
BURTON, PS [1 ]
机构
[1] UPJOHN CO,DRUG DELIVERY SYST RES,KALAMAZOO,MI 49001
关键词
PEPTIDE; TRANSPORT; PERMEABILITY; LIPOPHILICITY; HYDROGEN BONDING; CELL CULTURE;
D O I
10.1023/A:1015825912542
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The relationship between structure and permeability of peptides across epithelial cells was studied. Using confluent monolayers of Caco-2 cells as a model of the intestinal epithelium, permeability coefficients were obtained from the stead-state flux of a series of neutral and zwitterionic peptides prepared from D-phenylalanine and glycine. Although these peptides ranged in lipophilicity (log octanol/water partition coefficient) from -2.2 to +2.8, no correlation was found between the observed flux and the apparent lipophilicity. However, a strong correlation was found for the flux of the neutral series and the total number of hydrogen bonds the peptide could potentially make with water. These results suggest that a major impediment to peptide passive absorption is the energy required to break water-peptide hydrogen bonds in order for the solute to enter the cell membrane. This energy appears not to be offset by the favorable introduction of lipophilic side chains in the amino acid residues.
引用
收藏
页码:1453 / 1460
页数:8
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