AS HLA-DRW53-RESTRICTED T-CELL EPITOPE FROM A NOVEL MYCOBACTERIUM-LEPRAE PROTEIN ANTIGEN IMPORTANT TO THE HUMAN-MEMORY T-CELL REPERTOIRE AGAINST MYCOBACTERIUM-LEPRAE

Cellular immunity mediated by T cells plays a major role in protection against intracellular infections, including leprosy a chronic disease caused by Mycobacterium leprae. In this work, we describe CD4(+) T-cell clones, isolated from healthy humans immunized with M. leprae, which recognize a novel M. leprae protein antigen previously isolated from a lambda gt11 DNA expression library. On the basis of the deduced primary structure of the carboxyl-terminal part of the antigen, we have used a synthetic-peptide approach to exactly define the T-cell epitope recognized. Importantly, major histocompatibility complex restriction studies showed that the epitope is presented by an HLA-DRw53 molecule which is frequently expressed in many populations. In addition, we have demonstrated that a long-term cell-mediated immunity response against the peptide epitope is present after immunization with M. leprae. In conclusion, the M. leprae T-cell epitope described here fulfills the primary criteria for subunit vaccine candidates against leprosy.