EVIDENCE FOR RIFAMPICIN-PROMOTED READTHROUGH OF A FULLY RHO-DEPENDENT TRANSCRIPTIONAL TERMINATOR

被引：11

作者：

CROMIE, KD ^{[1
]}

HAYWARD, RS ^{[1
]}

机构：

[1] UNIV EDINBURGH, DEPT MOLEC BIOL, EDINBURGH EH9 3JR, MIDLOTHIAN, SCOTLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1984年 / 193卷 / 03期

关键词：

D O I：

10.1007/BF00382095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rifampicin promotes readthrough of various transcriptional terminators in vivo, a conclusion based on previous measurements of galactokinase or .beta.-galactosidase synthesis in Escherichia coli strains with plasmids having the terminators fused upstream of galK or lacZ, respectively. However, the terminators tested did not include a fully rho-dependent signal. It is now shown that the drug stimulates galactokinase production when galK is fused downstream of such a terminator, the 1st rightward terminator of phage .lambda.. The nusA1 host mutation affects neither termination nor the drug response at this site.

引用

页码：532 / 534

页数：3

共 16 条

[1] CONTROL OF TRANSCRIPTION TERMINATION
ADHYA, S
GOTTESMAN, M
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1978, 47 : 967 - 996
[2] Friedman D.I., 1971, BACTERIOPHAGE LAMBDA, P733
[3] EVIDENCE THAT A NUCLEOTIDE-SEQUENCE, BOXA, IS INVOLVED IN THE ACTION OF THE NUSA PROTEIN
FRIEDMAN, DI
OLSON, ER
[J]. CELL, 1983, 34 (01) : 143 - 149
[4] TERMINATION OF TRANSCRIPTION BY NUSA GENE PROTEIN OF ESCHERICHIA-COLI
GREENBLATT, J
MCLIMONT, M
HANLY, S
[J]. NATURE, 1981, 292 (5820) : 215 - 220
[5] REGULATION OF TRANSCRIPTION TERMINATION BY THE N-GENE PROTEIN OF BACTERIOPHAGE-LAMBDA
GREENBLATT, J
[J]. CELL, 1981, 24 (01) : 8 - 9
[6] HOROWITZ H, 1982, J BIOL CHEM, V257
[7] EFFECT OF RIFAMPICIN ON EXPRESSION OF LACZ FUSED TO PROMOTERS OR TERMINATORS OF THE ESCHERICHIA-COLI RPOBC OPERON
HOWE, KM
NEWMAN, AJ
GARNER, I
WALLIS, A
HAYWARD, RS
[J]. NUCLEIC ACIDS RESEARCH, 1982, 10 (22) : 7425 - 7438
[8] TRANSCRIPTION TERMINATES AT LAMBDA-TR1 IN 3 CLUSTERS
LAU, LF
ROBERTS, JW
WU, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (20): : 6171 - 6175
[9] CHARACTERIZATION OF THE CLONED TERMINATOR-TR1, TERMINATOR-TL3 AND TERMINATOR-TI AND THE NUT R ANTITERMINATION SITE OF COLIPHAGE-LAMBDA
LUK, KC
SZYBALSKI, W
[J]. GENE, 1982, 20 (02) : 127 - 134
[10] MCKENNEY K, 1981, GENE AMPLIFICATION A, V2

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