DYNAMIC MOTIONS OF 1,6-DIPHENYL-1,3,5-HEXATRIENE IN INTERDIGITATED C(18)-C(10)PHOSPHATIDYLCHOLINE BILAYERS

被引:21
作者
KAO, YL
CHONG, PLG
HUANG, CH
机构
[1] MEHARRY MED COLL,DEPT BIOCHEM,NASHVILLE,TN 37208
[2] UNIV VIRGINIA,SCH MED,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22908
关键词
D O I
10.1016/S0006-3495(90)82439-5
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This study investigates the dynamic behavior of 1,6-diphenyl-1,3,5-hexatriene (DPH) in C(18):C(10)phosphatidylcholine [C(18):C(10)PC] bilayers. C(18):C(10)PC is an asymmetric mixed-chain phosphatidylcholine known to form mixed-interdigitated structures below the transition temperature and form partially interdigitated bilayers above the transition temperature. The rotation of DPH in C(18):C(10)PC has been described in terms of the thermal coefficient of rotation using the modified Y-plot method which takes into account the limiting anisotropy value. During the phase transition of C(18):C(10)PC, DPH exhibits a thermal coefficient b2M = 0.41 - 0.51 degrees C-1 which is similar to the b2M values obtained with noninterdigitated phosphatidylcholine bilayers. Differential polarized phase-modulation fluorometry has also been employed to study the dynamic behavior of DPH in C(18):C(10)PC in real time. The data show that DPH contains considerable motion in the highly ordered mixed interdigitated bilayers. The DPH motion steadily increases with an increase in temperature as shown by the rotational correlation time, and the wobbling diffusion constant. However, the limiting anisotropy, the order parameter, and the width of the lifetime distribution undergo an abrupt decrease, and a corresponding abrupt increase in the cone angle, at approximately 16 degrees C. This temperature range is near the onset temperature of the phase transition as determined by differential scanning calorimetry. The rotational parameters show strong hysteresis on heating and cooling. All the rotational parameters derived from DPH fluorescence in mixed interdigitated C(18):C(10)PC exhibit magnitudes similar to those obtained from noninterdigitated gel phases of symmetric diacylphosphatidylcholines.(ABSTRACT TRUNCATED AT 250 WORDS) © 1990, The Biophysical Society. All rights reserved.
引用
收藏
页码:947 / 956
页数:10
相关论文
共 43 条
[1]   RESOLVABILITY OF FLUORESCENCE LIFETIME DISTRIBUTIONS USING PHASE FLUOROMETRY [J].
ALCALA, JR ;
GRATTON, E ;
PRENDERGAST, FG .
BIOPHYSICAL JOURNAL, 1987, 51 (04) :587-596
[2]   BINARY-MIXTURES OF SATURATED AND UNSATURATED MIXED-CHAIN PHOSPHATIDYLCHOLINES - A DIFFERENTIAL SCANNING CALORIMETRY STUDY [J].
ALI, S ;
LIN, HN ;
BITTMAN, R ;
HUANG, CH .
BIOCHEMISTRY, 1989, 28 (02) :522-528
[3]   EFFECT OF ORIENTATIONAL ORDER ON THE DECAY OF THE FLUORESCENCE ANISOTROPY IN MEMBRANE SUSPENSIONS - EXPERIMENTAL-VERIFICATION ON UNILAMELLAR VESICLES AND LIPID ALPHA-LACTALBUMIN COMPLEXES [J].
AMELOOT, M ;
HENDRICKX, H ;
HERREMAN, W ;
POTTEL, H ;
VANCAUWELAERT, F ;
VANDERMEER, W .
BIOPHYSICAL JOURNAL, 1984, 46 (04) :525-539
[4]   BEHAVIOR OF SPIN LABELS IN A VARIETY OF INTERDIGITATED LIPID BILAYERS [J].
BOGGS, JM ;
RANGARAJ, G ;
WATTS, A .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 981 (02) :243-253
[5]   CALORIMETRIC STUDIES OF THE EFFECTS OF CHOLESTEROL ON THE PHASE-TRANSITION OF C(18) - C(10) PHOSPHATIDYLCHOLINE [J].
CHONG, PL ;
CHOATE, D .
BIOPHYSICAL JOURNAL, 1989, 55 (03) :551-556
[6]   DEPOLARIZATION OF DEHYDROERGOSTEROL IN PHOSPHOLIPID-BILAYERS [J].
CHONG, PLG ;
THOMPSON, TE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 863 (01) :53-62
[7]   TRANSVERSE LOCATION OF THE FLUORESCENT-PROBE 1,6-DIPHENYL-1,3,5-HEXATRIENE IN MODEL LIPID BILAYER-MEMBRANE SYSTEMS BY RESONANCE EXCITATION-ENERGY TRANSFER [J].
DAVENPORT, L ;
DALE, RE ;
BISBY, RH ;
CUNDALL, RB .
BIOCHEMISTRY, 1985, 24 (15) :4097-4108
[8]   FLUORESCENCE LIFETIME DISTRIBUTIONS OF 1,6-DIPHENYL-1,3,5-HEXATRIENE IN PHOSPHOLIPID-VESICLES [J].
FIORINI, R ;
VALENTINO, M ;
WANG, S ;
GLASER, M ;
GRATTON, E .
BIOCHEMISTRY, 1987, 26 (13) :3864-3870
[9]  
Flory P.J., 1989, STAT MECH CHAIN MOL
[10]   A CONTINUOUSLY VARIABLE FREQUENCY CROSS-CORRELATION PHASE FLUOROMETER WITH PICOSECOND RESOLUTION [J].
GRATTON, E ;
LIMKEMAN, M .
BIOPHYSICAL JOURNAL, 1983, 44 (03) :315-324