MOLECULAR-CLONING OF CDNA FOR VITAMIN-D3 25-HYDROXYLASE FROM RAT-LIVER MITOCHONDRIA

被引：94

作者：

USUI, E

NOSHIRO, M

OKUDA, K

机构：

[1] Department of Biochemistry, Hiroshima University School of Dentistry, Hiroshima

来源：

FEBS LETTERS | 1990年 / 262卷 / 01期

关键词：

5β-Cholestane-3α; 7α; 12α-triol; 27-hydroxylase; cDNA cloning; Cytochrome P-450; Vitamin D[!sub]3[!/sub] 25-hydroxylase;

D O I：

10.1016/0014-5793(90)80172-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding mitochondrial vitamin D3 25-hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 1.9 kbp long and contained a 1599 bp open reading frame encoding 533 amino acid residues. The deduced primary structure contained a presequence typical for mitochondrial enzymes in the N-terminal region. The N-terminal sequence of the mature enzyme was determined to be Ala-Ile-Pro-Ala-Ala, which agrees perfectly with a portion of the deduced sequence, establishing the cleavage point of the precursor. © 1990.

引用

页码：135 / 138

页数：4

共 20 条

[1]

ANDERSSON S, 1989, J BIOL CHEM, V264, P8222

[2]

ANDERSSON S, 1986, J BIOL CHEM, V261, P6932

[3]

BJORKHEM I, 1979, J BIOL CHEM, V254, P9518

[4]

BLACK SD, 1982, J BIOL CHEM, V257, P5927