TRANSCRIPTIONAL ANALYSIS OF THE GAP-PGK-TPI-PPC GENE-CLUSTER OF CORYNEBACTERIUM-GLUTAMICUM

被引：47

作者：

SCHWINDE, JW ^{[1
]}

THUMSCHMITZ, N ^{[1
]}

EIKMANNS, BJ ^{[1
]}

SAHM, H ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH, INST BIOTECHNOL 1, POSTFACH 1913, W-5170 JULICH 1, GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 12期

关键词：

D O I：

10.1128/JB.175.12.3905-3908.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcriptional organization of the Corynebacterium glutamicum gap-pgk-tpi-ppc gene cluster, encoding glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, triosephosphate isomerase, and phosphoenolpyruvate carboxylase, was investigated by Northern (RNA) blot and primer extension analyses. Four transcripts corresponding to gap, to gap-pgk-tpi, to pgk-tpi, and to pgk-tpi-ppc were identified. The respective transcriptional initiation sites in front of gap and pgk were located, and, from the analysis of DNA sequences upstream of these and of previously determined transcriptional start sites, common structures which may be important for promoter function in C. glutamicum are discussed.

引用

页码：3905 / 3908

页数：4

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