ERYTHROCYTE GALACTOKINASE ASSAY WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：8

作者：

MIZOGUCHI, N

EGUCHI, T

SAKURA, N

UEDA, K

机构：

[1] Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima

来源：

CLINICA CHIMICA ACTA | 1993年 / 216卷 / 1-2期

关键词：

GALACTOKINASE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); MASS SCREENING FOR GALACTOSEMIA; GALACTOKINASE DEFICIENCY;

D O I：

10.1016/0009-8981(93)90147-V

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

An assay for erythtocyte galactokinase based on high performance liquid chromatographic determination of galactose 1-phosphate (Gal-1-P) is described. The determination of Gal-1-P was applied to a post-column fluorometric detection of reducing sugars using arginine. This method is as sensitive and accurate as conventional radioisotopic methods, but needs no radioisotopic facilities. It requires only a small blood sample and is suitable as a follow-up test in neonatal screening.

引用

页码：145 / 151

页数：7

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