REACTIVITIES OF HIV-1 GAG-DERIVED PEPTIDES WITH ANTIBODIES OF HIV-1-INFECTED AND UNINFECTED HUMANS

被引：18

作者：

HAIST, S ^{[1
]}

MARZ, J ^{[1
]}

WOLF, H ^{[1
]}

MODROW, S ^{[1
]}

机构：

[1] UNIV REGENSBURG,FRANZ JOSEF STRAUSS ALLEE,W-8400 REGENSBURG,GERMANY

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1992年 / 8卷 / 11期

关键词：

D O I：

10.1089/aid.1992.8.1909

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A group of 41 peptides, each 24 amino acids long and overlapping with each other by 12 residues spanning the total gag open reading frame (orf) of HIV-1 (HTLV-III(BH) 10 isolate) were synthesized using Fmoc chemistry. The purified compounds were used in ELISA assays and tested for antibody reactivities in sera of human HIV-1-infected and noninfected individuals. Sera of HIV humans showed reactivity against four defined regions, two in p 1 7, one in p24, and one in p 1 5. The values of these reactivities were elevated especially in serum samples of HIV- individuals showing cross-reaction with gag proteins on Western blot. Amino acid sequence comparison of HIV-1 gag proteins with those of human endogenous retroviruses (ERV K10, ERV 3) revealed significant similarities predominantly in the domains showing elevated antibody cross-reactions. The majority of sera from HIV-1+ individuals showed strong reactivities to the cross-reactive regions and to various other peptide sequences, a sequential epitope recognized by all HIV-1+ sera could, however, not be identified. The results suggest that human individuals may have immune reactions to endogenous retroviral protein sequences, which are enhanced by infections with HIV-1. Specific antibodies to HIV-1 gag proteins are probably mainly directed to tertiary structure defined epitopes formed by particle formation of the p24 monomers to the nucleocapsid.

引用

页码：1909 / 1917

页数：9

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