THE PRODUCTION AND CHARACTERIZATION OF A NEW MONOCLONAL-ANTIBODY TO THE TREFOIL PEPTIDE HUMAN SPASMOLYTIC POLYPEPTIDE

被引：40

作者：

ELIA, G ^{[1
]}

CHINERY, R ^{[1
]}

HANBY, AM ^{[1
]}

POULSOM, R ^{[1
]}

WRIGHT, NA ^{[1
]}

机构：

[1] ROYAL COLL SURGEONS ENGLAND,IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,LONDON WC2A 3PN,ENGLAND

来源：

HISTOCHEMICAL JOURNAL | 1994年 / 26卷 / 08期

关键词：

D O I：

10.1007/BF00158289

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.

引用

页码：644 / 647

页数：4

共 12 条

[1] LOCALIZATION OF INTESTINAL TREFOIL-FACTOR MESSENGER-RNA IN RAT STOMACH AND INTESTINE BY HYBRIDIZATION INSITU [J].

CHINERY, R ;

POULSOM, R ;

ROGERS, LA ;

JEFFERY, RE ;

LONGCROFT, JM ;

HANBY, AM ;

WRIGHT, NA .

BIOCHEMICAL JOURNAL, 1992, 285 :5-8

[2] EXPRESSION AND PURIFICATION OF A TREFOIL PEPTIDE MOTIF IN A BETA-GALACTOSIDASE FUSION PROTEIN AND ITS USE TO SEARCH FOR TREFOIL-BINDING SITES [J].

CHINERY, R ;

POULSOM, R ;

ELIA, G ;

HANBY, AM ;

WRIGHT, NA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (02) :557-563

[3] SPASMOLYTIC POLYPEPTIDE IS A MAJOR ANTRAL PEPTIDE - DISTRIBUTION OF THE TREFOIL PEPTIDES HUMAN SPASMOLYTIC POLYPEPTIDE AND PS2 IN THE STOMACH [J].

HANBY, AM ;

POULSOM, R ;

SINGH, S ;

ELIA, G ;

JEFFERY, RE ;

WRIGHT, NA .

GASTROENTEROLOGY, 1993, 105 (04) :1110-1116

[4]

HANBY AM, 1993, AM J PATHOL, V142, P663

[5] PNR-2 PS2 IMMUNOHISTOCHEMICAL STAINING IN BREAST-CANCER - CORRELATION WITH PROGNOSTIC FACTORS AND ENDOCRINE RESPONSE [J].

HENRY, JA ;

PIGGOTT, NH ;

MALLICK, UK ;

NICHOLSON, S ;

FARNDON, JR ;

WESTLEY, BR ;

MAY, FEB .

BRITISH JOURNAL OF CANCER, 1991, 63 (04) :615-622

[6] GROWTH STIMULATORY EFFECT OF PANCREATIC SPASMOLYTIC POLYPEPTIDE ON CULTURED COLON AND BREAST-TUMOR CELLS [J].

HOOSEIN, NM ;

THIM, L ;

JORGENSEN, KH ;

BRATTAIN, MG .

FEBS LETTERS, 1989, 247 (02) :303-306

[7] SPASMOLYTIC POLYPEPTIDE - A TREFOIL PEPTIDE SECRETED BY RAT GASTRIC MUCOUS CELLS [J].

JEFFREY, GP ;

OATES, PS ;

WANG, TC ;

BABYATSKY, MW ;

BRAND, SJ .

GASTROENTEROLOGY, 1994, 106 (02) :336-345

[8] PANCREATIC SPASMOLYTIC POLYPEPTIDE (PSP) .3. PHARMACOLOGY OF A NEW PORCINE PANCREATIC-POLYPEPTIDE WITH SPASMOLYTIC AND GASTRIC-ACID SECRETION INHIBITORY EFFECTS [J].

JORGENSEN, KD ;

DIAMANT, B ;

JORGENSEN, KH ;

THIM, L .

REGULATORY PEPTIDES, 1982, 3 (3-4) :231-243

[9] TREFOIL PEPTIDES - A NEWLY RECOGNIZED FAMILY OF EPITHELIAL MUCIN-ASSOCIATED MOLECULES [J].

POULSOM, R ;

WRIGHT, NA .

AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02) :G205-G213

[10]

POULSOM R, 1992, SCAND J GASTROENTE S, V192, P17

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