INDUCIBLE GENE-EXPRESSION OF ACTIVIN-A ERYTHROID-DIFFERENTIATION FACTOR IN HL-60 CELLS

Treatment of HL-60 cells with 12-O-tetradecanoyl-phorbol 13-acetate (TPA) for 48 h induced expression of mRNA of βA chain of activin A/erythroid differentiation factor. Under the same condition, interferon-γ caused a slight increase in βA chain mRNA, whereas 1α, 25-dihydroxyvitamin D3, dimethylsulfoxide and all-trans-retinoic acid failed to induce this mRNA in HL-60 cells. Furthermore, 4 h- treatment with TPA or lipopolysaccharide (LPS) induced a marked increase in βA chain mRNA levels in interferon-γ-pretreated HL-60 cells. In the cells pretreated with 1α, 25-dihydroxyvitamin D3, TPA and LPS induced as little increase in βA chain mRNA as in the control cells. Neither α nor βB chain mRNA was detected in any sample. These results indicate that interferon-γ has a priming effect on the activation of activin A/erythroid differentiation factor gene by TPA or LPS in HL-60 cells. © 1990.