EVALUATION OF ANALYTICAL METHODS - LIQUID-CHROMATOGRAPHY OF CEFALEXIN

被引：5

作者：

HENDRIX, C ^{[1
]}

ZHU, YX ^{[1
]}

VANHOUTVEN, C ^{[1
]}

THOMAS, J ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,INST FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM LAB,VAN EVENSTRAAT 4,B-3000 LOUVAIN,BELGIUM

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1993年 / 100卷 / 1-3期

关键词：

CEFALEXIN; QUANTITATIVE ANALYSIS; LIQUID CHROMATOGRAPHY; METHOD EVALUATION;

D O I：

10.1016/0378-5173(93)90093-U

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A comparative study of three isocratic liquid chromatographic methods for the analysis of cefalexin is described. Two methods, taken from the European Pharmacopoeia and the US Pharmacopeia, use classical alkyl bonded phases (C18) as the stationary phase. The third method uses poly(styrene-divinylbenzene). Poor reproducibility of the selectivity towards cefalexin and its related substances was observed for the C18 columns. In both C18 methods, none of the columns was able to separate all the potential related substances from cefalexin under the prescribed LC conditions. The copolymer columns on the other hand showed good selectivity, independent of the origin or age of the columns. Four bulk samples of cefalexin were analysed by these three methods and the results were compared. Although the selectivity of polymer columns was better, for the samples examined no significant differences were observed for the assay results obtained with the three methods.

引用

页码：213 / 218

页数：6

共 5 条

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