A PROTEIN-KINASE-C INHIBITORY ACTIVITY IS PRESENT IN THE HUMAN T-LYMPHOBLAST CELL-LINE JURKAT

被引：6

作者：

COLUMELLI, S ^{[1
]}

HOULLIER, AM ^{[1
]}

PERIGNON, JL ^{[1
]}

机构：

[1] UNIV PARIS 05,BIOCHIM LAB,INSERM,U75,156 RUE VAUGIRARD,F-75730 PARIS 15,FRANCE

来源：

IMMUNOLOGY LETTERS | 1991年 / 30卷 / 03期

关键词：

D O I：

10.1016/0165-2478(91)90041-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The human lymphoblast cell line Jurkat is widely used as a model system for studying signal transduction pathways during lymphocyte activation. We report the presence of a potent endogenous inhibitor of protein kinase C (PKC) in the cytosolic fraction of Jurkat cells. This inhibitor is not diffusible and is thermolabile; it is assumed to be a protein. It was separated from PKC by ion-exchange chromatography on DEAE-cellulose. The inhibitory activity was partially reversed by increasing the concentration of the PKC substrate; increasing that of PKC activators (calcium and phospholipids) was without effect. PKC activity was inhibited by more than 90% in the crude cytosolic fraction but the inhibition could be completely reversed by diluting the cell extract. This inhibitory activity could not be detected in the cytosol from normal lymphocytes or from lymphoblasts from leukemic patients.

引用

页码：297 / 300

页数：4

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