HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AND TYPE-2 PROTEASE MONOMERS ARE FUNCTIONALLY INTERCHANGEABLE IN THE DIMERIC ENZYMES

被引：11

作者：

PATTERSON, CE ^{[1
]}

SEETHARAM, R ^{[1
]}

KETTNER, CA ^{[1
]}

CHENG, YSE ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT CO,WILMINGTON,DE 19880

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 02期

关键词：

D O I：

10.1128/JVI.66.2.1228-1231.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human immunodeficiency virus type 1 (HIV-1) and HIV-2 proteases are dimers of identical subunits. We made a construct for the expression of recombinant one-chain HIV-2 protease dimer, which, like the previously described one-chain HIV-1 protease dimer, is fully active. The constructs for the one-chain dimers of HIV-1 and HIV-2 proteases were modified to produce hybrid one-chain dimers consisting of both HIV-1 and HIV-2 protease monomers. Although the monomers share only 47.5% sequence identity, the hybrid one-chain dimers are fully active, suggesting that the folding of both HIV-1 and HIV-2 protease monomers is functionally similar.

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页码：1228 / 1231

页数：4

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