ANALYSIS OF POLAR LIPIDS FROM SOME REPRESENTATIVE ENTEROBACTERIA, PLESIOMONAS AND ACINETOBACTER BY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

Fast atom bombardment-mass spectrometry (FAB-MS) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. The following micro-organisms were tested: Acinetobacter calcoaceticus, Acinetobacter sp., Citrobacter freundii, Enterobacter cloacae (2 strains), Escherichia coli (3 strains), Hafnia alvei, Klebsiella oxytoca, Klebsiella pneumoniae, Morganella morganii, Plesiomonas shigelloides, Proteus mirabilis (3 strains), Serratia liquefaciens and Serratia marcescens. Negative-ion spectra provide data for twenty-seven major carboxylate anions (m/z 209-325) and for thirty-seven major phospholipid anions (m/z 645-774). Generally, the largest carboxylate peaks were due to 16 : 1, 16 : 0, cyc17 and 18 : 1 while the largest phospholipid anion peaks were due to PE(32 : 1), PE(33 : 1), PE(34 : 1), PE(34 : 2), PG(30 : 2), PG(31 : 2), PG(32 : 2), PG(34 : 1) and PS (33 : 0). However, quantitative differences were observed. For example, Acinetobacter lacked PE (33 : 1) but had exceptionally high peaks at m/z 748, PS(33 : 0), and m/z 281, octadecanoate. Unknown 'carboxylate' peaks were detected at m/z 254, 256, 261, 268, 282 and 301. In some cases, unknown peaks appeared to constitute possible homologous series being separated by DELTA m/z of 14(= methylene). For chemotaxonomic purposes, the complexity of the data required numerical analysis. Using the Pearson coefficient of linear correlation, as a measure of association, it was possible to compare all strains analysed. Typical results for strain comparisons were as follows: Ent. cloacae vs Ent. cloacae, r = 0.90 (Ent. cloacae vs Ac. calcoaceticus, r = 0.46). Thus FAB-MS represents an excellent means of obtaining large quantities of data on polar lipids of a range of bacterial isolates, which may be suitable for chemotaxonomic purposes.