SEPARATION OF MONO-PHASE, DI-PHASE, AND TRISTEARIN FROM AN INDUSTRIAL MIXTURE OF GLYCERIDES BY NORMAL-PHASE AND REVERSE-PHASE HPLC

This work reports on a quantitative and qualitative study of reverse and normal-phase HPLC to separate mono-, di-, and tristearin from an industrial mixture of glycerides. For normal-phase chromatography a CN-bonded packed column was used, while C8 and C18 columns were used for reverse-phase chromatography. Both types of chromatography were studied with UV and RI detection. The influence of some chromatographic factors, such as the bonded phase, mobile-phase composition, and flow rate, on the retention times, selectiveness, and effectiveness were studied.