VISIBLE DIODE-LASER INDUCED FLUORESCENCE DETECTION IN LIQUID-CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION OF THIOLS

被引：40

作者：

MANK, AJG

MOLENAAR, EJ

LINGEMAN, H

GOOIJER, C

BRINKMAN, UAT

VELTHORST, NH

机构：

[1] Department of General and Analytical Chemistry, Free University, NL-1081 HV Amsterdam

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 17期

关键词：

D O I：

10.1021/ac00065a006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

New cyanine labels containing only a single active group for the chemical derivatization of thiols were synthesized in order to apply visible diode laser induced fluorescence (DIO-LIF) detection in conventional-size liquid chromatography (LC). 2-Mercaptobenzothiazole (MBT) was selected as test compound. The most appropriate label was CY5.4a-IA, a sulfonated dicarbocyanine with a iodoacetamide reactive group. The detection limit of labeled MBT in the LC-DIO-LIF system was 8 X 10(-12) M. The detection limit of MBT was 1 x 10(-9) M; lower concentrations are not labeled quantitatively. The injection repeatability was 1.0% (n = 6), the reaction repeatability was 3.0% (n = 6), and linearity was observed from 2.5 X 10(-9) to 1.0 x 10(-5) M. As an example, MBT-spiked river water and urine samples were derivatized with CY5.4a-IA and analyzed by LC-DIO-LIF. Hardly any interference was found. The method compares very favorably with existing techniques.

引用

页码：2197 / 2203

页数：7

共 21 条

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