EFFECT OF GYPSOGENIN 3,O-GLUCURONIDE PRETREATMENT OF GYPSOPHILA-PANICULATA AND SAPONARIA-OFFICINALIS CELL-SUSPENSION CULTURES ON THE ACTIVITIES OF MICROSOMAL 2,3-OXIDOSQUALENE CYCLOARTENOL AND AMYRIN CYCLASES

被引:14
作者
HENRY, M [1 ]
RAHIER, A [1 ]
TATON, M [1 ]
机构
[1] INST BIOL MOLEC PLANTES,DEPT ENZYMOL CELLULAIRE & MOLEC,CNRS,UPR 406,F-67083 STRASBOURG,FRANCE
关键词
GYPSOPHILA-PANICULATA; SAPONARIA-OFFICINALIS; CARYOPHYLLACEAE; CELL SUSPENSION CULTURES; BIOSYNTHESIS; PENTACYCLIC TRITERPENOIDAL SAPONINS; GYPSOGENIN; 3; O-GLUCURONIDE; AMYRINS; CYCLOARTENOL; PHYTOSTEROLS;
D O I
10.1016/S0031-9422(00)97541-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The regulation of triterpenoid saponin biosynthesis in Gypsophila paniculata and Saponaria officinalis suspension cells has been investigated by studying the effects of adding gypsogenin 3,O-glucuronide, a ubiquitous saponin precursor in plants of the genus Gypsophila which contain gypsogenin saponins, to the cultures. Treatment of suspended G. paniculata cells with gypsogenin 3,O-glucuronide 24 hr before administration of [C-14]acetate resulted in a marked reduction in the incorporation of radioactivity into saponins and their precursors but not into sterols and steryl glucosides. 2,3-Oxidosqualene-cycloartenol and -amyrin cyclase activities were measured in microsomal fractions obtained from suspension cultures of the two species. Pretreatment of cultures with gypsogenin 3,O-glucuronide was shown to lead to inhibition (in G. paniculata cells) or stimulation (in S. officinalis cells) of the 2,3-oxidosqualene-amyrin cyclase activity and was without any marked effect on 2,3-oxidosqualene-cycloartenol cyclase activity. These results strongly suggest that in these species 2,3-oxidosqualene-cyclases are regulating steps in the isoprenoid pathway orienting the biosynthetic flux towards either tetracyclic or pentacyclic triterpenes.
引用
收藏
页码:3855 / 3859
页数:5
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