ESTABLISHMENT OF A PHYLOGENETIC SURVEY SYSTEM FOR AIDS-RELATED LENTIVIRUSES AND DEMONSTRATION OF A NEW HIV-2 SUBGROUP

被引：54

作者：

MIURA, T

SAKURAGI, J

KAWAMURA, M

FUKASAWA, M

MORIYAMA, EN

GOJOBORI, T

ISHIKAWA, K

MINGLE, JAA

NETTEY, VBA

AKARI, H

ENAMI, M

TSUJIMOTO, H

HAYAMI, M

机构：

[1] KYOTO UNIV,INST VIRUS RES,IMMUNODEFICIENCY VIRUS RES CTR,KYOTO 606,JAPAN

[2] YAMANASHI INST PUBL HLTH,DEPT BIOL,YAMANASHI,JAPAN

[3] NATL INST GENET,DEPT EVOLUTIONARY GENET,MISHIMA,SHIZUOKA 411,JAPAN

[4] UNIV TOKYO,DEPT VET INTERNAL MED,TOKYO 113,JAPAN

[5] NATL INST HLTH,DEPT MEASLES VIRUS,TOKYO 141,JAPAN

[6] ST JOSEPH HOSP,KOFORIDUA,GHANA

[7] UNIV GHANA,NOGUCHI MEM INST MED RES,LEGON,GHANA

来源：

AIDS | 1990年 / 4卷 / 12期

关键词：

AIDS; HIV; SIMIAN IMMUNODEFICIENCY VIRUS; PHYLOGENETIC TREE; LENTIVIRUS; POLYMERASE CHAIN REACTION;

D O I：

10.1097/00002030-199012000-00012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIV(mac)/SIV(sm) in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

引用

页码：1257 / 1261

页数：5

共 44 条

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