PARTIAL-PURIFICATION OF 3-ALPHA-HYDROXYSTEROID AND 3-BETA-HYDROXYSTEROID DEHYDROGENASES FROM HUMAN HYPERPLASTIC PROSTATE - COMPARISON BETWEEN THE 2 ENZYMES

被引：7

作者：

AMET, Y ^{[1
]}

SIMON, B ^{[1
]}

QUEMENER, E ^{[1
]}

MANGIN, P ^{[1
]}

FLOCH, HH ^{[1
]}

ABALAIN, JH ^{[1
]}

机构：

[1] CHU MORVAN,SERV UROL,F-29200 BREST,FRANCE

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1992年 / 41卷 / 3-8期

关键词：

D O I：

10.1016/0960-0760(92)90405-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3-alpha-Hydroxysteroid dehydrogenase (3-alpha-HSD) activity has been purified to homogeneity, the enzyme is a monomer with a M(w) of 32,000 Da. 3-beta-Hydroxysteroid dehydrogenase (3-beta-HSD) activity has been partially purified and has an apparent M(w) of 30,000 Da. Both enzymes have the same cofactor requirements, optimal pH. However 3-beta-HSD appeared to be an integral protein dependent on protein environment for its activity while 3-alpha-HSD activity is a protein more loosely associated to membranes.

引用

页码：689 / 692

页数：4

共 14 条

[1] METABOLISM OF ANDROGENS IN HUMAN HYPERPLASTIC PROSTATE - EVIDENCE FOR A DIFFERENTIAL LOCALIZATION OF THE ENZYMES INVOLVED IN THE METABOLISM
ABALAIN, JH
QUEMENER, E
CARRE, JL
SIMON, B
AMET, Y
MANGIN, P
FLOCH, HH
[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1989, 34 (1-6) : 467 - 471
[2] SELECTIVE RETENTION OF DIHYDROTESTO-STERONE BY PROSTATIC NUCLEI
ANDERSON, KM
LIAO, S
[J]. NATURE, 1968, 219 (5151) : 277 - &
[3] SOME ASPECTS OF MECHANISM OF ACTION OF STEROID-HORMONES
BAULIEU, EE
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1975, 7 (03) : 157 - 174
[4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5] BRUCHOVSKY N, 1968, J BIOL CHEM, V243, P2012
[6] PURIFICATION AND CHARACTERIZATION OF NADP+-DEPENDENT 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM MOUSE-LIVER CYTOSOL
HARA, A
INOUE, Y
NAKAGAWA, M
NAGANEO, F
SAWADA, H
[J]. JOURNAL OF BIOCHEMISTRY, 1988, 103 (06) : 1027 - 1034
[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[8] EFFECT OF ANDROGENIC HORMONES ON REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE - DELTA-4-3-KETOSTEROID 5 ALPHA-OXIDOREDUCTASE OF RAT VENTRAL PROSTATE
MOORE, RJ
WILSON, JD
[J]. ENDOCRINOLOGY, 1973, 93 (03) : 581 - 592
[9] CATABOLISM OF STEROIDS-C-19 BY SUBCELLULAR-FRACTIONS OF MAMMALIAN AND AVIAN TISSUES .4. EVALUATION OF SPECIFIC ACTIVITY OF BIOCHEMICALLY-PREPARED CARRIER CRYSTALLIZED ANDROGEN RADIOMETABOLITES BY A NEW PROCEDURE
MORFIN, RF
BERTHOU, F
FLOCH, HH
VENA, RL
OFNER, P
[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1973, 4 (04) : 381 - 391
[10] PENNING TM, 1985, J BIOL CHEM, V260, P5266

← 1 2 →