CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF MANDELONITRILE LYASE FROM ALMONDS

被引：24

作者：

LAUBLE, H ^{[1
]}

MULLER, K ^{[1
]}

SCHINDELIN, H ^{[1
]}

FORSTER, S ^{[1
]}

EFFENBERGER, F ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST KRISTALLOG,D-14195 BERLIN,GERMANY

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1994年 / 19卷 / 04期

关键词：

HYDROXYNITRILE LYASE; FLAVOENZYME; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1002/prot.340190410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals of three different isoenzymes of (R)-(+) mandelonitrile lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have been obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 69.9, b = 95.1, c = 95.6 Angstrom, and beta = 118.5 degrees. A complete set of diffraction data has been collected to 2.6 Angstrom resolution on native crystals of isoenzyme III. (C) 1994 Wiley-Liss, Inc.

引用

页码：343 / 347

页数：5

共 22 条

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