Differentiation of Acinetobacter calcoaceticus sensu stricto from related Acinetobacter species by electrophoretic polymorphism of malate dehydrogenase, glutamate dehydrogenase and catalase

被引:6
作者
Bouvet, PJM
Jeanjean, S
机构
[1] Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur
关键词
Acinetobacter; Acinetobacter calcoaceticus sensu stricto; malate dehydrogenase; glutamate dehydrogenase; catalase; enzyme polymorphism; A-baumannii; electrophoresis; taxonomy;
D O I
10.1016/0923-2508(96)81073-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acinetobacter baumannii, unnamed Acinetobacter species 3 (studied by P.J.M. Bouvet and P.A.D. Grimont) and unnamed DNA group 13 (studied by I. Tjernberg and J. Ursing) are the most prevalent Acinetobacter species in hospitals. Using the identification scheme of Bouvet and Grimont, it is sometimes difficult to differentiate these species from A. calcoaceticus sensu stricto, a species of the natural environment that has seldom been found associated with human infection. Genetically identified Acinetobacter isolates belonging to A. calcoaceticus sensu stricto (n=12), A. baumannii (n=22), Acinetobacter species 3 (n=15) and DNA group 13 of Tjernberg and Ursing (n=26), Acinetobacter species 10 (n=2), Acinetobacter species 11 (n=2) and 3 strains ungrouped by DNA-DNA hybridization were investigated for electrophoretic separations of L-malate dehydrogenase (MDH), glutamate dehydrogenase (GDH) and catalase (CAT). All A. calcoaceticus sensu stricto isolates were easily differentiated from those of other species investigated by their high MDH values (relative mobility (Rf)=78), their low GDH values (Rf range: 24-28) and CAT values (Rf range: 34-42). Acinetobacter species 3 was differentiated from A. baumannii and DNA group 13 of Tjernberg and Ursing by high CAT values. A. baumannii could not be differentiated from Tjernberg and Ursing DNA group 13. Acinetobacter species 10 was clearly differentiated from Acinetobacter species 11. Once an Acinetobacter is phenotypically identified with the four closely related species investigated here, electrophoretic analysis of MDH, GDH and CAT might be a useful complement to the identification scheme of Bouvet and Grimont for accurately identifying A, calcoaceticus sensu stricto.
引用
收藏
页码:773 / 785
页数:13
相关论文
共 35 条
[1]   ZONE ELECTROPHORESIS OF ENZYMES IN BACTERIAL TAXONOMY [J].
BAPTIST, JN ;
SHAW, CR ;
MANDEL, M .
JOURNAL OF BACTERIOLOGY, 1969, 99 (01) :180-&
[2]   AN UNDERESTIMATED NOSOCOMIAL PATHOGEN, ACINETOBACTER-CALCOACETICUS [J].
BERGOGNEBEREZIN, E ;
JOLYGUILLOU, ML .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1985, 16 (05) :535-537
[3]   PHENOTYPIC CHARACTERIZATION OF ACINETOBACTER STRAINS OF 13 DNA-DNA HYBRIDIZATION GROUPS BY MEANS OF THE BIOLOG SYSTEM [J].
BERNARDS, AT ;
DIJKSHOORN, L ;
VANDERTOORN, J ;
BOCHNER, BR ;
VANBOVEN, CPA .
JOURNAL OF MEDICAL MICROBIOLOGY, 1995, 42 (02) :113-119
[4]   DELINEATION OF NEW PROTEOLYTIC GENOMIC SPECIES IN THE GENUS ACINETOBACTER [J].
BOUVET, PJM ;
JEANJEAN, S .
RESEARCH IN MICROBIOLOGY, 1989, 140 (4-5) :291-299
[5]   TAXONOMY OF THE GENUS ACINETOBACTER WITH THE RECOGNITION OF ACINETOBACTER-BAUMANNII SP-NOV, ACINETOBACTER-HAEMOLYTICUS SP-NOV, ACINETOBACTER-JOHNSONII SP-NOV, AND ACINETOBACTER-JUNII SP-NOV AND EMENDED DESCRIPTIONS OF ACINETOBACTER-CALCOACETICUS AND ACINETOBACTER-LWOFFII [J].
BOUVET, PJM ;
GRIMONT, PAD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1986, 36 (02) :228-240
[6]   SPECIES, BIOTYPE, AND BACTERIOPHAGE TYPE DETERMINATIONS COMPARED WITH CELL-ENVELOPE PROTEIN PROFILES FOR TYPING ACINETOBACTER STRAINS [J].
BOUVET, PJM ;
JEANJEAN, S ;
VIEU, JF ;
DIJKSHOORN, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (02) :170-176
[7]   IDENTIFICATION AND BIOTYPING OF CLINICAL ISOLATES OF ACINETOBACTER [J].
BOUVET, PJM ;
GRIMONT, PAD .
ANNALES DE L INSTITUT PASTEUR-MICROBIOLOGIE, 1987, 138 (05) :569-578
[8]   ESCHERICHIA-VULNERIS - A NEW SPECIES OF ENTEROBACTERIACEAE ASSOCIATED WITH HUMAN WOUNDS [J].
BRENNER, DJ ;
MCWHORTER, AC ;
KNUTSON, JKL ;
STEIGERWALT, AG .
JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (06) :1133-1140
[9]   USE OF A SINGLE-STRAND SPECIFIC NUCLEASE FOR ANALYSIS OF BACTERIAL AND PLASMID DEOXYRIBONUCLEIC ACID HOMODUPLEXES AND HETERODUPLEXES [J].
CROSA, JH ;
BRENNER, DJ ;
FALKOW, S .
JOURNAL OF BACTERIOLOGY, 1973, 115 (03) :904-911
[10]   NUMERICAL-ANALYSIS OF CELL-ENVELOPE PROTEIN PROFILES OF ACINETOBACTER STRAINS CLASSIFIED BY DNA-DNA HYBRIDIZATION [J].
DIJKSHOORN, L ;
TJERNBERG, I ;
POT, B ;
MICHEL, MF ;
URSING, J ;
KERSTERS, K .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1990, 13 (04) :338-344