INTERMEDIATE FILAMENTS IN CARDIAC MYOGENESIS - NESTIN IN THE DEVELOPING MOUSE HEART

被引：131

作者：

KACHINSKY, AM ^{[1
]}

DOMINOV, JA ^{[1
]}

MILLER, JB ^{[1
]}

机构：

[1] MASSACHUSETTS GEN HOSP,NEUROMUSCULAR LAB,BOSTON,MA 02129

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1995年 / 43卷 / 08期

关键词：

NESTIN; INTERMEDIATE FILAMENT; HEART DEVELOPMENT; CARDIOMYOCYTE; MOUSE; REVERSE TRANSCRIPTASE PCR; IMMUNOHISTOCHEMISTRY;

D O I：

10.1177/43.8.7542682

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

By using immunohistology combined with immunoblotting, cell. culture, and RT-PCR, we show that the intermediate filament protein nestin is transiently expressed in the midembryonic mouse heart. Monoclonal antibody (MAb) Rat-401, known to react with nestin in neural and skeletal muscle cells, was also found to react with ventricular and atrial cells throughout the mouse heart from embryonic day 9 (E9) through E10.5. Both before (E8.5) and after (E11-adult) this brief period, staining with Rat-401 was absent from atrial. and ventricular myocytes. To evaluate the specificity of staining with MAb Rat-401 in the heart, we used immunoblotting, cell culture, and RT-PCR to verify that the authentic nestin protein and mRNA were expressed in cardiomyocytes of the E10 mouse. Nestin expression is the first molecular marker for this distinct midembryonic period of heart development.

引用

页码：843 / 847

页数：5

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