DNA METAL-BINDING BY ANTITUMOR-ACTIVE METALLOCENE DICHLORIDES FROM INDUCTIVELY COUPLED PLASMA SPECTROSCOPY ANALYSIS - TITANOCENE DICHLORIDE FORMS DNA CP2TI OR DNA CPTI ADDUCTS DEPENDING ON PH

被引：87

作者：

MCLAUGHLIN, ML ^{[1
]}

CRONAN, JM ^{[1
]}

SCHALLER, TR ^{[1
]}

SNELLING, RD ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,BASIN RES INST,BATON ROUGE,LA 70803

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1990年 / 112卷 / 24期

关键词：

D O I：

10.1021/ja00180a046

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Inductively coupled plasma (ICP) spectroscopy is used to measure the DNA-metal binding of the antitumor agents Cp2TiCI2, Cp2VCl2, Cp2NbCI2, and cis-(H3N)2PtCl2 and of Cp2ZrCl2 and Cp2HfCl2 in 10 or 110 mM sodium perchlorate with an initial phosphorus to metal ratio of 10:1. All the metals of these complexes bind DNA except vanadium from vanadocene dichloride. There is no release of metal when isolated DNA-metal adduct is dissolved in fresh 10 mM sodium perchlorate for up to 48 h. DNA binding studies using 3H-labeled titanocene dichloride are consistent with a DNA-Cp2Ti adduct at pH 5.3 and a DNA-CpTi adduct at pH 7.0 A DNA-titanium adduct is also formed from CpTiCl3 and DNA in 10 mM sodium perchlorate with an initial phosphorus to metal ratio of 20:1. © 1990, American Chemical Society. All rights reserved.

引用

页码：8949 / 8952

页数：4

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