DNA FINGERPRINTS APPLIED TO PATERNITY ANALYSIS IN APPLES (MALUS X DOMESTICA)

被引：81

作者：

NYBOM, H ^{[1
]}

SCHAAL, BA ^{[1
]}

机构：

[1] WASHINGTON UNIV,DEPT BIOL,ST LOUIS,MO 63130

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 79卷 / 06期

关键词：

Apple; DNA fingerprint; M13; probe; Paternity analysis; RFLP;

D O I：

10.1007/BF00224242

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI. © 1990 Springer-Verlag.

引用

页码：763 / 768

页数：6

共 23 条

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