IMMUNOPEROXIDASE LOCALIZATION OF PNEUMOCYSTIS-CARINII IN FORMALIN FIXED, PARAFFIN EMBEDDED TISSUE WITH MONOCLONAL ANTIBODY-2G2

被引：1

作者：

CARTUN, RW

LACHMAN, MF

PEDERSEN, CA

COLE, SR

KOVACS, JA

机构：

[1] Anatomic Pathology Division, Department of Pathology, Hartford Hospital, Hartford, CT

[2] Department of Critical Care Medicine, Clinical Center, National Institutes of Health, Bethesda, MD

来源：

JOURNAL OF HISTOTECHNOLOGY | 1990年 / 13卷 / 02期

关键词：

Immunoperoxidase; Monoclonal antibody 2G2; Pneumocystis carinii;

D O I：

10.1179/his.1990.13.2.117

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

An immunocytochemical method for identifying Pneumocystis carinii (P. carinii) in formalin fixed, paraffin embedded tissue is described. The procedure utilized a commercially available monoclonal antibody and a sensitive avidin-biotin detection system. The 2G2 P. carinii antibody produced intense labeling of cyst walls, extra-cystic fibrillar material, and trophozoites, even in tissue that had been fixed in formalin for over 2 years. The procedure identified 30 of 30 known P. carinii infected lung tissues. Immunolabeled P. carinii organisms were easily visible under low power magnification. No false positives were observed. No immunoreactivity was observed with a variety of normal tissues and various fungi. The availability of a commercial antibody that reliably and specifically identifies P. carinii in histologically processed tissues will benefit pathologists, clinicians, and researchers. (The J Histotechnol 13:117, 1990). © 1990 Maney Publishing.

引用

页码：117 / 120

页数：4

共 16 条

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