TRANS SPLICING OF NEMATODE PRE-MESSENGER-RNA INVITRO

被引：84

作者：

HANNON, GJ

MARONEY, PA

DENKER, JA

NILSEN, TW

机构：

[1] Department of Molecular Biology, Microbiology Case Western Reserve University School of Medicine Cleveland

来源：

CELL | 1990年 / 61卷 / 07期

关键词：

D O I：

10.1016/0092-8674(90)90689-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5′ terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mRNA at an authentic trans splice acceptor site. SL addition occurs via a trans splicing reaction that proceeds through Y-branched intermediates. The branch-point is located at either of two adenosine residues located 18 and 19 nucleotides upstream of the splice acceptor site. © 1990.

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页码：1247 / 1255

页数：9

相关论文

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