DIFFERENTIATION OF ANCHORING JUNCTIONS IN TRACHEAL BASAL CELLS IN THE GROWING RAT

被引:10
作者
EVANS, MJ
COX, RA
BURKE, AS
MOLLER, PC
机构
[1] UNIV TEXAS, MED BRANCH, DEPT HUMAN BIOL CHEM & GENET, GALVESTON, TX 77550 USA
[2] SHRINERS BURN INST, GALVESTON, TX 77550 USA
关键词
D O I
10.1165/ajrcmb/6.2.153
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A function of airway basal cells is to attach ciliated and nonciliated columnar cells to the basal lamina. The significance of the basal cell in attachment is related to the height of the columnar epithelium. In taller epithelia, basal cells are more numerous and differentiated with respect to anchoring junctional adhesion mechanisms (desmosomes, hemidesmosomes, and the cytoskeleton) than in shorter epithelia. In this study, we determined if basal cell anchoring junctional adhesion mechanisms differentiated during growth of the airway. Tracheas from five 3-day-old, five 30-day-old, and five 90-day-old rats were prepared for electron microscopy and morphometrically studied by standard techniques. The circumference of the trachea increased from 2.5 +/- 0.2 to 7.5 +/- 0.4 mm during growth. The height of the columnar cell increased from 13.4 +/- 1.5 to 24.6 +/- 3.9-mu-m, and the number of basal cells per millimeter increased from 3.2 +/- 0.7 to 9.6 +/- 1.8 during growth. The number of desmosomes per basal cell profile increased significantly from 1.5 +/- 0.1 to 2.1 +/- 0.1, as did keratin filament volume density from 0.046 +/- 0.05 to 0.098 +/- 0.032. The amount of hemidesmosome attachment per basal cell did not increase significantly during growth of the airway. These data demonstrate that as tracheas grow in circumference, the columnar cells increase in height, basal cells increase in number, and anchoring junctional adhesion mechanisms differentiate in the basal cells. These changes are closely related to the height of the epithelium and result in maintaining a constant amount of attachment between the columnar epithelium and the basal lamina as the epithelium increases in height.
引用
收藏
页码:153 / 157
页数:5
相关论文
共 15 条
  • [1] BENNETT M, 1989, MOL BIOL CELL, P791
  • [2] JUNCTIONAL ADHESION MECHANISMS IN AIRWAY BASAL CELLS
    EVANS, MJ
    COX, RA
    SHAMI, SG
    PLOPPER, CG
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1990, 3 (04) : 341 - 347
  • [3] THE ROLE OF BASAL CELLS IN ADHESION OF COLUMNAR EPITHELIUM TO AIRWAY BASEMENT-MEMBRANE
    EVANS, MJ
    PLOPPER, CG
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 138 (02): : 481 - 483
  • [4] BIOLOGY OF AIRWAY BASAL CELLS
    EVANS, MJ
    MOLLER, PC
    [J]. EXPERIMENTAL LUNG RESEARCH, 1991, 17 (03) : 513 - 531
  • [5] THE ROLE OF BASAL CELLS IN ATTACHMENT OF COLUMNAR CELLS TO THE BASAL LAMINA OF THE TRACHEA
    EVANS, MJ
    COX, RA
    SHAMI, SG
    WILSON, B
    PLOPPER, CG
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1989, 1 (06) : 463 - 469
  • [6] Franke WW, 1986, JUNCTIONAL COMPLEXES, P26
  • [7] GARROD DR, 1986, J CELL SCI, P221, DOI 10.1242/jcs.1986.Supplement_4.14
  • [8] DISTRIBUTION OF NON-CILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELLS IN INTRAPULMONARY AND EXTRA-PULMONARY AIRWAYS OF THE RABBIT
    PLOPPER, CG
    HALSEBO, JE
    BERGER, WJ
    SONSTEGARD, KS
    NETTESHEIM, P
    [J]. EXPERIMENTAL LUNG RESEARCH, 1983, 5 (02) : 79 - 98
  • [9] COMPARISON OF NON-CILIATED TRACHEAL EPITHELIAL-CELLS IN 6 MAMMALIAN-SPECIES - ULTRASTRUCTURE AND POPULATION-DENSITIES
    PLOPPER, CG
    MARIASSY, AT
    WILSON, DW
    ALLEY, JL
    NISHIO, SJ
    NETTESHEIM, P
    [J]. EXPERIMENTAL LUNG RESEARCH, 1983, 5 (04) : 281 - 294
  • [10] DIFFERENTIATION OF TRACHEAL EPITHELIUM DURING FETAL LUNG MATURATION IN THE RHESUS-MONKEY MACACA-MULATTA
    PLOPPER, CG
    ALLEY, JL
    WEIR, AJ
    [J]. AMERICAN JOURNAL OF ANATOMY, 1986, 175 (01): : 59 - 71