DNA STRETCHING ON FUNCTIONALIZED GOLD SURFACES

被引：94

作者：

ZIMMERMANN, RM

COX, EC

机构：

[1] Department of Molecular Biology, Princeton University, Princeton

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 03期

关键词：

D O I：

10.1093/nar/22.3.492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au - biotin - streptavidin biotin - DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.

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页码：492 / 497

页数：6

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