THE AMINO-ACID-SEQUENCE PREVIOUSLY ATTRIBUTED TO A PROTEIN-KINASE OR A TCP1-RELATED MOLECULAR CHAPERONE AND COPURIFIED WITH PHYTOCHROME IS A BETA-GLUCOSIDASE

被引：16

作者：

GUSMAYER, S

BRUNNER, H

SCHNEIDERPOETSCH, HAW

LOTTSPEICH, F

ECKERSKORN, C

GRIMM, R

RUDIGER, W

机构：

[1] UNIV MUNICH,INST BOT,D-80638 MUNICH,GERMANY

[2] UNIV COLOGNE,INST BOT,D-50931 COLOGNE,GERMANY

[3] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

[4] HEWLETT PACKARD CORP,D-76337 WALDBRONN,GERMANY

来源：

FEBS LETTERS | 1994年 / 347卷 / 01期

关键词：

AVENA SATIVA; AVENACOSIDASE; BETA-GLUCOSIDASE AGGREGATE; PHYTOCHROME;

D O I：

10.1016/0014-5793(94)00503-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 60 kDa protein (P60) co-purified with phytochrome was identified as avenacosidase, a beta-glucosidase which is part of the defense system of Avena sativa. An antiserum raised against P60 was used to isolate a cDNA clone coding for the complete amino acid sequence of P60. The cDNA-derived amino acid sequence contained the partial sequences described before for a protein kinase [(1989) Planta 178, 199-206] and for a TCP1-related molecular chaperone [(1993) Nature 363, 644-647] co-purified with phytochrome. We conclude that these activities were related to minor contaminants and that only sequences of avenacosidase had been obtained.

引用

页码：51 / 54

页数：4

共 13 条

[1] RELEASE OF ACTIVE CYTOKININ BY A BETA-GLUCOSIDASE LOCALIZED TO THE MAIZE ROOT-MERISTEM [J].