IDENTIFICATION OF CDNA CLONES CODING FOR THE STYLE SPECIFIC S11A-GLYCOPROTEIN GENE ASSOCIATED WITH GAMETOPHYTIC SELF-INCOMPATIBILITY IN TOMATO (LYCOPERSICON-PERUVIANUM)

被引:9
作者
CHUNG, IK [1 ]
NAKATA, K [1 ]
TANAKA, H [1 ]
ITO, T [1 ]
HORIUCHI, H [1 ]
OHTA, A [1 ]
TAKAGI, M [1 ]
机构
[1] KAGOME RES INST, TOCHIGI 32929, JAPAN
关键词
D O I
10.1271/bbb.57.1172
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We isolated 18 cDNA candidates that were expected to encode parts of the self-incompatibility-associated RNase of Lycopersicon peruvianum PI 126441 (S11a-plant) from a style cDNA library. Polymerase chain reaction was used with oligonucleotides derived from conserved amino acid sequences of RNases from fungi and S-associated RNases in other species of Solanaceae. Two of these clones (both do not have full length sequences) gave 826-bp and 730-bp sequences, and they had the same open reading frame, named ORF-1. Comparison of the deduced amino acid sequence of ORF-1 with S-associated RNase of other Solanaceous plants showed a high degree of similarity. mRNA encoding this ORF-1 was about 1000 bases long and detected only in the style tissue by Northern analysis. Using one of the cDNA clones as a probe, we detected sequence variability among three different S-genotypes of randomly chosen wild-type tomatoes by Southern analysis. From these results, it was concluded that ORF-1 encodes the self-incompatibility associated S-glycoprotein of L. peruvianum PI 126441 (S11a-plant).
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页码:1172 / 1176
页数:5
相关论文
共 19 条
  • [1] SEQUENCE VARIABILITY OF 3 ALLELES OF THE SELF-INCOMPATIBILITY GENE OF NICOTIANA-ALATA
    ANDERSON, MA
    MCFADDEN, GI
    BERNATZKY, R
    ATKINSON, A
    ORPIN, T
    DEDMAN, H
    TREGEAR, G
    FERNLEY, R
    CLARKE, AE
    [J]. PLANT CELL, 1989, 1 (05) : 483 - 491
  • [2] CLONING OF CDNA FOR A STYLAR GLYCOPROTEIN ASSOCIATED WITH EXPRESSION OF SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
    ANDERSON, MA
    CORNISH, EC
    MAU, SL
    WILLIAMS, EG
    HOGGART, R
    ATKINSON, A
    BONIG, I
    GREGO, B
    SIMPSON, R
    ROCHE, PJ
    HALEY, JD
    PENSCHOW, JD
    NIALL, HD
    TREGEAR, GW
    COGHLAN, JP
    CRAWFORD, RJ
    CLARKE, AE
    [J]. NATURE, 1986, 321 (6065) : 38 - 44
  • [3] CHUNG IK, 1992, INT S MOL BIOL TOMAT, P23
  • [4] SEQUENCE VARIABILITY AND DEVELOPMENTAL EXPRESSION OF S-ALLELES IN SELF-INCOMPATIBLE AND PSEUDO-SELF-COMPATIBLE PETUNIA
    CLARK, KR
    OKULEY, JJ
    COLLINS, PD
    SIMS, TL
    [J]. PLANT CELL, 1990, 2 (08) : 815 - 826
  • [5] de Nettancourt D., 1977, INCOMPATIBILITY ANGI
  • [6] GENETIC-POLYMORPHISM OF SELF-INCOMPATIBILITY IN FLOWERING PLANTS
    EBERT, PR
    ANDERSON, MA
    BERNATZKY, R
    ALTSCHULER, M
    CLARKE, AE
    [J]. CELL, 1989, 56 (02) : 255 - 262
  • [7] GRAY JE, 1991, PLANT CELL, V3, P271, DOI 10.1105/tpc.3.3.271
  • [8] GULBER U, 1983, GENE, V25, P263
  • [9] SELF-INCOMPATIBILITY - A SELF-RECOGNITION SYSTEM IN PLANTS
    HARING, V
    GRAY, JE
    MCCLURE, BA
    ANDERSON, MA
    CLARKE, AE
    [J]. SCIENCE, 1990, 250 (4983) : 937 - 941
  • [10] PRIMARY STRUCTURE OF A BASE NON-SPECIFIC RIBONUCLEASE FROM RHIZOPUS-NIVEUS
    HORIUCHI, H
    YANAI, K
    TAKAGI, M
    YANO, K
    WAKABAYASHI, E
    SANDA, A
    MINE, S
    OHGI, K
    IRIE, M
    [J]. JOURNAL OF BIOCHEMISTRY, 1988, 103 (03) : 408 - 418