MOLECULAR ELECTRONICS OF A SINGLE PHOTOSYSTEM-I REACTION-CENTER - STUDIES WITH SCANNING-TUNNELING-MICROSCOPY AND SPECTROSCOPY

被引：37

作者：

LEE, I ^{[1
]}

LEE, JW ^{[1
]}

WARMACK, RJ ^{[1
]}

ALLISON, DP ^{[1
]}

GREENBAUM, E ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,OAK RIDGE,TN 37831

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 06期

关键词：

D O I：

10.1073/pnas.92.6.1965

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Thylakoids and photosystem I (PSI) reaction centers were imaged by scanning tunneling microscopy. The thylakoids were isolated from spinach chloroplasts, and PSI reaction centers were extracted from thylakoid membranes. Because thylakoids are relatively thick nonconductors, they were sputter-coated with Pd/Au before imaging. PSI photosynthetic centers and chemically platinized PSI were investigated without sputter-coating. They were mounted on flat gold substrates that had been treated with mercaptoacetic acid to help bind the proteins. With tunneling spectroscopy, the PSI centers displayed a semiconductor-like response with a band gap of 1.8 eV. Lightly platinized (platinized for 1 hr) centers displayed diode-like conduction that resulted in dramatic contrast changes between images taken with opposite bias voltages. The electronic properties of this system were stable under long-term storage.

引用

页码：1965 / 1969

页数：5

共 41 条

[1] SCANNING TUNNELING MICROSCOPY OF PHOTOSYNTHETIC REACTION CENTERS [J].