DNA CONCENTRATION AND LENGTH IN SPUTUM OF PATIENTS WITH CYSTIC-FIBROSIS DURING INHALATION WITH RECOMBINANT HUMAN DNASE

被引：60

作者：

BRANDT, T ^{[1
]}

BREITENSTEIN, S ^{[1
]}

VONDERHARDT, H ^{[1
]}

TUMMLER, B ^{[1
]}

机构：

[1] HANNOVER MED SCH,ZENTRUM KINDERHEILKUNDE,KLIN FORSCH GRP,D-30623 HANNOVER,GERMANY

来源：

THORAX | 1995年 / 50卷 / 08期

关键词：

DNASE; CYSTIC FIBROSIS; SPUTUM ANALYSIS;

D O I：

10.1136/thx.50.8.880

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background - The clinical benefit of the administration of aerosolised recombinant human DNase (rhDNase) on pulmonary function in patients with cystic fibrosis has already been demonstrated but the biochemical action of rhDNase on DNA in bronchial secretions in vivo has not yet been investigated. Methods - Sputum was collected from 135 patients with cystic fibrosis before and during treatment with aerosolised rhDNase and examined to ascertain DNA concentration and length by colorimetric assay and densitometry of gel separated DNA. Results - Treatment with rhDNase reduced the concentration and the size of extracellular DNA in the sputum. The median interquartile range of DNA length decreased from 0.5-2.6 kbp before treatment to 0.3-1.0 kbp during treatment. Conclusions - rhDNase was delivered to the secretions and was enzymatically active in vivo.

引用

页码：880 / 882

页数：3

共 14 条

[1] RECOMBINANT HUMAN DNASE INHALATION IN NORMAL SUBJECTS AND PATIENTS WITH CYSTIC-FIBROSIS - A PHASE-1 STUDY [J].

AITKEN, ML ;

BURKE, W ;

MCDONALD, G ;

SHAK, S ;

MONTGOMERY, AB ;

SMITH, A .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1992, 267 (14) :1947-1951

[2]

Boat TF., 1989, CYSTIC FIBROSIS META, V6th, P2649

[3] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

BURTON, K .

BIOCHEMICAL JOURNAL, 1956, 62 (02) :315-323

[4]

CANTOR RC, 1980, BIOPHYSICAL CHEM BEH, P1019

[5] ESTIMATION OF DEOXYRIBONUCLEIC ACID IN PRESENCE OF SIALIC ACID - APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS [J].

CROFT, DN ;

LUBRAN, M .

BIOCHEMICAL JOURNAL, 1965, 95 (03) :612-&

[6] EFFECT OF AEROSOLIZED RECOMBINANT HUMAN DNASE ON EXACERBATIONS OF RESPIRATORY SYMPTOMS AND ON PULMONARY-FUNCTION IN PATIENTS WITH CYSTIC-FIBROSIS [J].

FUCHS, HJ ;

BOROWITZ, DS ;

CHRISTIANSEN, DH ;

MORRIS, EM ;

NASH, ML ;

RAMSEY, BW ;

ROSENSTEIN, BJ ;

SMITH, AL ;

WOHL, ME .

NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (10) :637-642

[7] ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM MAMMALIAN-CELLS [J].

GROSS-BELLARD, MM ;

OUDET, P ;

CHAMBON, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 36 (01) :32-38

[8] A PRELIMINARY-STUDY OF AEROSOLIZED RECOMBINANT HUMAN DEOXYRIBONUCLEASE-I IN THE TREATMENT OF CYSTIC-FIBROSIS [J].

HUBBARD, RC ;

MCELVANEY, NG ;

BIRRER, P ;

SHAK, S ;

ROBINSON, WW ;

JOLLEY, C ;

WU, M ;

CHERNICK, MS ;

CRYSTAL, RG .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (12) :812-815

[9] PUS, DEOXYRIBONUCLEIC-ACID, AND SPUTUM VISCOSITY [J].

PICOT, R ;

DAS, I ;

REID, L .

THORAX, 1978, 33 (02) :235-242

[10] EFFICACY AND SAFETY OF SHORT-TERM ADMINISTRATION OF AEROSOLIZED RECOMBINANT HUMAN DEOXYRIBONUCLEASE IN PATIENTS WITH CYSTIC-FIBROSIS [J].

RAMSEY, BW ;

ASTLEY, SJ ;

AITKEN, ML ;

BURKE, W ;

COLIN, AA ;

DORKIN, HL ;

EISENBERG, JD ;

GIBSON, RL ;

HARWOOD, IR ;

SCHIDLOW, DV ;

WILMOTT, RW ;

WOHL, ME ;

MEYERSON, LJ ;

SHAK, S ;

FUCHS, H ;

SMITH, AL .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1993, 148 (01) :145-151

← 1 2 →