DEMONSTRATION OF IMMUNO-BOUND ALKALINE-PHOSPHATASE BY A DIRECT LEAD METHOD - OPTIMIZATION OF REACTION PARAMETERS BY MICRODENSITOMETRY USING COMPUTER-AIDED IMAGE-PROCESSING

被引：3

作者：

BOCK, R ^{[1
]}

CHAKRAVERTYWERNER, K ^{[1
]}

HERTH, G ^{[1
]}

LIU, N ^{[1
]}

OSTERMANN, E ^{[1
]}

TOX, U ^{[1
]}

机构：

[1] TONGJI MED UNIV,DEPT HISTOL & EMBRYOL,WUHAN,PEOPLES R CHINA

来源：

ACTA HISTOCHEMICA | 1991年 / 91卷 / 02期

关键词：

D O I：

10.1016/S0065-1281(11)80277-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antigens labelled by the immunohistochemical alkaline phosphatase anti-alkaline phosphatase (APAAP) method can be visualized by a lead capture technique. Optimal conditions of the reaction, evaluated by microdensitometry using computer-aided image processing, are described.

引用

页码：201 / 211

页数：11

共 17 条

[1] QUANTITATIVE IMMUNOCYTOCHEMISTRY OF TYROSINE-HYDROXYLASE IN RAT-BRAIN .1. DEVELOPMENT OF A COMPUTER-ASSISTED METHOD USING THE PEROXIDASE-ANTIPEROXIDASE TECHNIQUE
BENNO, RH
TUCKER, LW
JOH, TH
REIS, DJ
[J]. BRAIN RESEARCH, 1982, 246 (02) : 225 - 236
[2] STAINABILITY OF NEUROSECRETORY MATERIAL AND BEGINNING OF ITS FORMATION IN SUPRAOPTICO-HYPOPHYSIAL SYSTEM
BOCK, R
BRINKMANN, H
MARCKWORT, W
[J]. ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE, 1968, 87 (04): : 534 - +
[3] SYNTHESIS, TRANSPORT, AND RELEASE OF POSTERIOR PITUITARY-HORMONES
BROWNSTEIN, MJ
RUSSELL, JT
GAINER, H
[J]. SCIENCE, 1980, 207 (4429) : 373 - 378
[4] IMMUNOENZYMATIC LABELING OF MONOCLONAL-ANTIBODIES USING IMMUNE-COMPLEXES OF ALKALINE-PHOSPHATASE AND MONOCLONAL ANTI-ALKALINE PHOSPHATASE (APAAP COMPLEXES)
CORDELL, JL
FALINI, B
ERBER, WN
GHOSH, AK
ABDULAZIZ, Z
MACDONALD, S
PULFORD, KAF
STEIN, H
MASON, DY
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (02) : 219 - 229
[5] FIRTH JA, 1976, HISTOCHEMISTRY, V47, P1454
[6] HOPE DB, 1974, HDB PHYSIOLOGY 1, V4, P173
[7] A DIRECT LEAD METHOD FOR ELECTRON MICROSCOPIC VISUALIZATION OF ALKALINE PHOSPHATASE ACTIVITY
HUGON, J
BORGERS, M
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1966, 14 (05) : 429 - &
[8] JOHANSSON O, 1985, WENNER GREN INT S SE, V42, P231
[9] MASON DY, 1985, TECH IMMUNOCYTOCHEM, V3, P25
[10] HISTOCHEMICAL DEMONSTRATION OF ACTIVITY OF PHOSPHATASES BY MEANS OF LEADRHODIZONATE
MEIJER, AEF
[J]. HISTOCHEMIE, 1966, 6 (04): : 317 - &

← 1 2 →