CHIRAL SEPARATIONS - A COMPARISON OF HPLC AND TLC

被引：19

作者：

DUNCAN, JD

机构：

[1] Department of Chemistry, University of Missouri, Rolla

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 14期

关键词：

D O I：

10.1080/01483919008049068

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparisons are made for separations attained in normal-phase HPLC and TLC involving N-carbobenzyloxy-glycyl-L-proline and (+/-)- 10-camphorsulfonic acid as chiral mobile phase additives/chiral counter ions (CMA). Possible reasons for differences in stereoselectivity of β-cyclodextrin CMA and chiral stationary phases (CSP) are discussed. In addition, differences in solubility and proposed separation mechanisms for native cyclodextrins (CD) versus derivatized CD are discussed. Inherent differences in high performance liquid chromatographic (HPLC) and thin-layer chromatographic (TLC) methods are outlined. The pros and cons of using HPLC and TLC are considered. © 1990, Taylor & Francis Group, LLC. All rights reserved.

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收藏

页码：2737 / 2755

页数：19

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