RIBOSOME FUNCTION DETERMINED BY FLUORESCENCE

被引：9

作者：

HARDESTY, B ^{[1
]}

ODOM, OW ^{[1
]}

PICKING, W ^{[1
]}

机构：

[1] UNIV TEXAS,CLAYTON FDN RES,AUSTIN,TX 78712

来源：

BIOCHIMIE | 1992年 / 74卷 / 04期

基金：

美国国家科学基金会;

关键词：

PROTEIN SYNTHESIS; RIBOSOMES; TRANSFER RNA; FLUORESCENCE;

D O I：

10.1016/0300-9084(92)90117-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five different fluorescence phenomena are considered in relation to their use to study the structure and function of ribosomes. These are: quantum yield or emission intensity; emission wavelength maximum; fluorescence anisotropy; collisional quenching; and nonradiative energy transfer. Results from a number of studies in which these techniques were used are described and summarized in relation to the movement and conformation of tRNA, the nascent peptide, and mRNA in a ribosome during the reaction steps of peptide elongation.

引用

页码：391 / 401

页数：11

共 34 条

[1] ELONGATING RIBOSOMES INVIVO ARE REFRACTORY TO ERYTHROMYCIN [J].