EXPRESSION OF HAPTOGLOBIN RECEPTORS IN HUMAN HEPATOMA-CELLS

被引:23
作者
OKUDA, M [1 ]
TOKUNAGA, R [1 ]
TAKETANI, S [1 ]
机构
[1] KANSAI MED UNIV,DEPT HYG,MORIGUCHI,OSAKA 570,JAPAN
关键词
HAPTOGLOBIN RECEPTOR; HEMOGLOBIN; HEME OXYGENASE; (HUMAN HEPATOMA CELL); (HUMAN HEPATOCYTE);
D O I
10.1016/0167-4889(92)90249-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The uptake of radio-labeled hemoglobin-haptglobin complex (Hb-Hp) by human hepatoma PLC/PRF/5 and HepG2 cells was investigated in an attempt to characterize the uptake process and intracellular transport. Human hepatoma cells took up Hb-Hp in a receptor-mediated manner. Scatchard analysis of binding revealed that PLC/PRF/5 and HepG2 cells exhibited about 21000 and 63000 haptoglobin receptors/cell, with a dissociation constant (K(d)) of 8.0 and 17 nM, respectively. Human hepatocytes in primary culture also expressed about 84000 receptors/cells, with a K(d) of 7.4 nM. The hemoglobin-haptoglobin complex was internalized and subsequently the internalized Hb-Hp was slowly degraded in the cells. Preincubation of the cells with Hb-Hp resulted in a decrease in binding of the radioactive Hb-Hp to the cell surface, and was accompanied with an accumulation of intracellular receptors. The uptake of Hb-Hp by the cells was not inhibited by 100-mu-M chloroquine or by 10 mM methylamine, but was inhibited by 50-mu-M monodansylcadaverine. Hemoglobin-heme taken up by the cells induced microsomal heme oxygenase. Thus, human hepatoma PLC/PRF/5 and HepG2 cells can take up Hb-Hp by haptoglobin receptor-mediated endocytosis and Hb-Hp probably causes translocation of the haptoglobin receptors from the cell surface to the cell interior where they can be degraded. The internalized heme-moiety of hemoglobin can regulate the expression of heme oxygenase.
引用
收藏
页码:143 / 149
页数:7
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