A NOVEL QUANTITATIVE RECEPTOR AUTORADIOGRAPHY AND IN-SITU HYBRIDIZATION HISTOCHEMISTRY TECHNIQUE USING STORAGE PHOSPHOR SCREEN IMAGING

被引：30

作者：

ITO, T ^{[1
]}

SUZUKI, T ^{[1
]}

LIM, DK ^{[1
]}

WELLMAN, SE ^{[1
]}

HO, IK ^{[1
]}

机构：

[1] UNIV MISSISSIPPI,MED CTR,DEPT PHARMACOL & TOXICOL,JACKSON,MS 39216

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1995年 / 59卷 / 02期

关键词：

STORAGE PHOSPHOR SCREEN; DIGITAL IMAGING; RECEPTOR AUTORADIOGRAPHY; S-35] T-BUTYLBICYCLOPHOSPHOROTHIONATE; DENSITOMETRY; IN SITU HYBRIDIZATION HISTOCHEMISTRY;

D O I：

10.1016/0165-0270(94)00212-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new technique of image acquisition for quantitative receptor autoradiography and in situ hybridization histochemistry was developed using storage phosphor screen imaging. This method was at least 4-5 times faster than conventional film densitometry. Two of the advantages of the phosphor screen method are high sensitivity and wide linear range of response. Other aspects of this method were compared with those of conventional densitometry. Use of storage phosphor screen imaging will allow greatly increased speed of pharmacological screening procedures that utilize quantitative autoradiography.

引用

页码：265 / 271

页数：7

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