NUCLEOTIDE-SEQUENCE OF ALPHA-GALACTOSIDASE CDNA FROM MORTIERELLA-VINACEA

被引：23

作者：

SHIBUYA, H

KOBAYASHI, H

KASAMO, K

KUSAKABE, I

机构：

[1] MINIST AGR FORESTRY & FISHERIES, NATL FOOD RES INST, IBARAKI 305, OSAKA, JAPAN

[2] UNIV TSUKUBA, INST APPL BIOCHEM, IBARAKI, OSAKA 305, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期

关键词：

D O I：

10.1271/bbb.59.1345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To analyze the primary structure of Mortierella vinacea alpha-galactosidase, a cDNA library of M. vinacea mRNA in lambda gt10 was constructed. A clone, which has an insert size of about 1.4 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence showed that the mature enzyme consisted of 397 amino acid residues with a molecular mass of 44,350Da. The sequence identity of the mature enzyme with alpha-galactosidases from Saccharomyces carlsbergensis, Cyamopsis tetragonoloba (guar), and human were 47%, 43%, and 34%, respectively.

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页码：1345 / 1348

页数：4

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