TETRAHYMENA-THERMOPHILA - GROWTH IN SYNTHETIC NUTRIENT MEDIUM IN THE PRESENCE AND ABSENCE OF GLUCOSE

被引：46

作者：

SZABLEWSKI, L

ANDREASEN, PH

TIEDTKE, A

FLORINCHRISTENSEN, J

FLORINCHRISTENSEN, M

RASMUSSEN, L

机构：

[1] ODENSE UNIV INST MOLEC BIOL,DK-5230 ODENSE,DENMARK

[2] INST ZOOL,W-4400 MUNSTER,GERMANY

[3] NATL UNIV LA PLATA,CTR PARASITE & VECTOR RES,RA-1900 LA PLATA,ARGENTINA

[4] ODENSE UNIV,INST MED BIOL,DK-5230 ODENSE,DENMARK

来源：

JOURNAL OF PROTOZOOLOGY | 1991年 / 38卷 / 01期

关键词：

ABSENCE OF GLUCOSE; GROWTH RATES; SYNTHETIC NUTRIENT MEDIUM;

D O I：

10.1111/j.1550-7408.1991.tb04802.x

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

This report contains the newest directions for preparation of the synthetic nutrient medium for some Tetrahymena species that do not require lipids. In the standard medium T. thermophila, strains SB 210 and 281, multiply at 37-degrees-C with doubling times of around 2 h and at 26-degrees-C around 5 h. We have established multiplication rates as functions of variations in the composition of the medium. In media in which all components are present at one-third of the normal concentrations and only the essential amino acids are included, growth and multiplication become sharply dependent on glucose in strain SB 281. Such media may be used for selection and enrichment of certain specified cell lines.

引用

页码：62 / 65

页数：4

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