A SMALL METALLORIBOZYME WITH A 2-STEP MECHANISM

被引：200

作者：

PAN, T

UHLENBECK, OC

机构：

[1] Department of Chemistry and Biochemistry, University of Colorado, Boulder

来源：

NATURE | 1992年 / 358卷 / 6387期

关键词：

D O I：

10.1038/358560a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.

引用

页码：560 / 563

页数：4

共 19 条

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