ULTRASOUND-AMPLIFIED IMMUNOHISTOCHEMISTRY

被引:14
作者
PODKLETNOVA, I [1 ]
ALHO, H [1 ]
机构
[1] UNIV TAMPERE,DEPT BIOMED SCI,POB 607,SF-33101 TAMPERE,FINLAND
关键词
ULTRASONIC IRRADIATION; MICROWAVE IRRADIATION; DIAZEPAM BINDING INHIBITOR; IMMUNOHISTOCHEMISTRY; VIBRATOME SECTIONS;
D O I
10.1177/41.1.8417112
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We describe a novel technique for improving the sensitivity of immunofluorescence staining by use of ultrasonic irradiation. Free-floating vibratome sections from rat cerebellum were incubated with primary antiserum and simultaneously were briefly exposed to ultrasound (US) in a conventional ultrasound bath. After the US treatment, a conventional immunohistochemical method was employed. Two different antisera and two conventional immunohistochemical detection systems were tested. In all cases a 10-20-sec US treatment strengthened immunoreactivity considerably. Irradiated samples showed a good morphology compared with non-irradiated sections. Our results demonstrate that with ultrasonic treatment the dilutions of primary antibodies can be increased and the incubation times of primary antisera can be reduced. The ultrasonic method described here requires no special equipment. It is easy, reproducible, and it can be considered a new method for the enhancement at immunohisto- and cytochemical staining of free-floating vibratome sections.
引用
收藏
页码:51 / 56
页数:6
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