OPTIMIZATION OF SOLVENT COMPOSITION FOR HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

被引：16

作者：

BAYNE, CK ^{[1
]}

MA, CY ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV ANALYT CHEM,OAK RIDGE,TN 37831

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1987年 / 10卷 / 16期

关键词：

CHROMATOGRAPHIC ANALYSIS - Liquid;

D O I：

10.1080/01483918708077811

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a mixture of five solvents for the mobile phase was maximized for the separation of twelve structurally related B(a)P metabolites on normal phase HPTLC plates. Volume fractions (rather than absolute quantities) of the mobile phase solvents were constrained by upper bounds by considering solvent strength. These restrictions defined an experimental region that is an irregular polyhedron formed from a truncated 4-dimensional simplex. The optimization of the expected separation response over the experimental region was based on a second-order Scheffe polynomial estimated from twenty-five experimental runs.

引用

页码：3529 / 3546

页数：18

共 26 条

[1]

Cornell J.A., 1981, EXPT MIXTURES

[2] THE GEOMETRY OF CONSTRAINED MIXTURE EXPERIMENTS [J].