VISUALIZATION OF PROTEASES WITHIN A COMPLEX SAMPLE FOLLOWING THEIR SELECTIVE RETENTION ON IMMOBILIZED BACITRACIN, A PEPTIDE ANTIBIOTIC

被引：28

作者：

VANNOORT, JM

VANDENBERG, P

MATTERN, IE

机构：

[1] TNO-Medical Biological Laboratory, 2280 AA Rijswijk

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90444-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger. © 1991.

引用

页码：385 / 390

页数：6

共 22 条

[1]

AUNSTRUP K, 1980, ECON MICROBIOL, V5, P49

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] RAPID AND SENSITIVE DETECTION OF PROTEOLYTIC-ENZYMES AFTER ELECTROPHORESIS [J].