HIGHLY SENSITIVE DETECTION OF VIRAL-RNA GENOMES IN BLOOD SPECIMENS BY AN OPTIMIZED REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION

被引：13

作者：

MURAKAMI, S

TAKAHASHI, Y

YOSHIDA, S

FUKE, I

OHMAE, K

MORI, C

TAKAGI, M

TAKAMIZAWA, A

OKAYAMA, H

机构：

[1] OSAKA UNIV,RES FDN MICROBIAL DIS,KANONJI INST,KAGAWA 768,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT BIOCHEM,TOKYO 113,JAPAN

来源：

JOURNAL OF MEDICAL VIROLOGY | 1994年 / 43卷 / 02期

关键词：

HEPATITIS C VIRUS; JAPANESE ENCEPHALITIS VIRUS; POLYMERASE CHAIN REACTION; BLOOD SPECIMENS; HIGHLY SENSITIVE DETECTION;

D O I：

10.1002/jmv.1890430215

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested polymerase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription-polymerase chain reaction (ORTPCR) detects as few as 3-5 virions in 0.1 ml of whole blood specimens. The effectiveness of this assay system is confirmed by diagnosis of human hepatitis C viral (HCV) infection. (C) 1994 Wiley-Liss, Inc.

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页码：175 / 181

页数：7

相关论文

共 26 条

[1] DETECTION OF CYTOMEGALO-VIRUS IN URINE FROM NEWBORNS BY USING POLYMERASE CHAIN-REACTION DNA AMPLIFICATION [J].

DEMMLER, GJ ;

BUFFONE, GJ ;

SCHIMBOR, CM ;

MAY, RA .

JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (06) :1177-1184

[2]

Dulbecco R, 1988, VIROLOGY, P1

[3] VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITTS LYMPHOMA [J].

EPSTEIN, MA ;

ACHONG, BG ;

BARR, YM .

LANCET, 1964, 1 (733) :702-+

[4] NON-A, NON-B HEPATITIS IN CHIMPANZEES AND MARMOSETS [J].

FEINSTONE, SM ;

ALTER, HJ ;

DIENES, HP ;

SHIMIZU, Y ;

POPPER, H ;

BLACKMORE, D ;

SLY, D ;

LONDON, WT ;

PURCELL, RH .

JOURNAL OF INFECTIOUS DISEASES, 1981, 144 (06) :588-598

[5] DETECTION OF REPLICATIVE INTERMEDIATES OF HEPATITIS-C VIRAL-RNA IN LIVER AND SERUM OF PATIENTS WITH CHRONIC HEPATITIS-C [J].

FONG, TL ;

SHINDO, M ;

FEINSTONE, SM ;

HOOFNAGLE, JH ;

DIBISCEGLIE, AM .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03) :1058-1060

[6] DETECTION OF MEASLES-VIRUS GENOMIC SEQUENCES IN SSPE BRAIN-TISSUE BY THE POLYMERASE CHAIN-REACTION [J].

GODEC, MS ;

ASHER, DM ;

SWOVELAND, PT ;

ELDADAH, ZA ;

FEINSTONE, SM ;

GOLDFARB, LG ;

GIBBS, CJ ;

GAJDUSEK, DC .

JOURNAL OF MEDICAL VIROLOGY, 1990, 30 (04) :237-244

[7] BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION .12. PLAQUING EFFICIENCIES OF PURIFIED HUMAN ADENOVIRUSES [J].

GREEN, M ;

PINA, M ;

KIMES, RC .

VIROLOGY, 1967, 31 (03) :562-&

[8] DETECTION OF PHOCID DISTEMPER VIRUS-RNA IN SEAL TISSUES USING SLOT HYBRIDIZATION AND THE POLYMERASE CHAIN-REACTION AMPLIFICATION ASSAY - GENETIC-EVIDENCE THAT THE VIRUS IS DISTINCT FROM CANINE-DISTEMPER VIRUS [J].

HAAS, L ;

SUBBARAO, SM ;

HARDER, T ;

LIESS, B ;

BARRETT, T .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :825-832

[9] DNA TYPING FROM SINGLE HAIRS [J].

HIGUCHI, R ;

VONBEROLDINGEN, CH ;

SENSABAUGH, GF ;

ERLICH, HA .

NATURE, 1988, 332 (6164) :543-546

[10] DETECTION AND QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS RNA IN PATIENT SERUM BY USE OF THE POLYMERASE CHAIN-REACTION [J].

HOLODNIY, M ;

KATZENSTEIN, DA ;

SENGUPTA, S ;

WANG, AM ;

CASIPIT, C ;

SCHWARTZ, DH ;

KONRAD, M ;

GROVES, E ;

MERIGAN, TC .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (04) :862-866