STUDIES ON SMALL (LESS-THAN-300 MU-M) MICROCAPSULES .2. PARAMETERS GOVERNING THE PRODUCTION OF ALGINATE BEADS BY HIGH-VOLTAGE ELECTROSTATIC PULSES

被引:61
作者
HALLE, JP
LEBLOND, FA
PARISEAU, JF
JUTRAS, P
BRABANT, MJ
LEPAGE, Y
机构
[1] UNIV MONTREAL,DEPT MATH & STAT,MONTREAL H3C 3J7,PQ,CANADA
[2] INST RECH ELECT QUEBEC,VARENNES J3X 1S1,PQ,CANADA
关键词
MICROENCAPSULATION; ALGINATE BEAD SIZE;
D O I
10.1177/096368979400300503
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The size of microcapsules is a critical parameter in the immunoisolation of islets of Langerhans by microencapsulation. The use of smaller capsules decreases the total implant volume and improves insulin kinetics and oxygen supply. A high voltage electrostatic pulse system was used for the production of small (<300 mu m) alginate beads, the first step of the encapsulation technique. However, islets often protruded from capsules that were too small, further emphasizing the need for a method to control bead size. A study of 7 parameters [electrostatic pulse amplitude (A), duration (D) and wavelength (lambda), pump flow rate (P), needle gauge, alginate viscosity and distance between electrodes] showed that P (r = 0.981, p = 0.003) and lambda (r = 0.988, p = 0.0002) were the principal determinants of bead size. To detect potential interactions between parameters, 270 combinations of different levels of A, D, lambda, and P were studied. A multivariate regression analysis of these data confirmed that P and lambda are the prime determinants of bead size, and showed that a 2-parameter (P, lambda) model could be used to precisely predict bead size (R(2) = 0.84), while keeping the application simple. The precision of the predictive model is only slightly improved by the use of additional parameters. The reliability of the data used to elaborate this model was demonstrated (p = 0.6226) by comparing them with a second data set obtained under the same conditions. A third set of experiments confirmed the applicability of the model. This work has major implications on the preclinical application of microencapsulation since it showed that it is possible to predetermine the bead size.
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页码:365 / 372
页数:8
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