INTERLEUKIN-1-STIMULATION AND TUMOR NECROSIS FACTOR-STIMULATION OF PROSTAGLANDIN-E2 SYNTHESIS IN MDCK CELLS, AND POTENTIATION OF THIS EFFECT BY CYCLOHEXIMIDE

被引：21

作者：

LEIGHTON, JD ^{[1
]}

PFEILSCHIFTER, J ^{[1
]}

机构：

[1] CIBA GEIGY AG, DEPT RES, R-1056-P-23, CH-4002 BASEL, SWITZERLAND

来源：

FEBS LETTERS | 1990年 / 259卷 / 02期

关键词：

Cycloheximide; Interleukin-1; Madin-Darby canine kidney cell; Prostaglandin-E2; TNFα;

D O I：

10.1016/0014-5793(90)80029-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of interleukin (IL)- lα, IL- 1β and TNFα on prostaglandin-E2 synthesis in Madin-Darby canine kidney (MDCK) cells were investigated. IL-lβ time- and dose-dependently stimulated prostaglandin-E2 synthesis. While TNFα produced a comparatively small but significant stimulation of PGE2 release, coincubation of IL-lβ with TNFα produced a marked synergistic stimulation of PGE2 release. The effect of IL-lβ and of IL-1β and TNFα was apparent as early as after 2 h of incubation. The enhanced PGE2 synthesis was inhibited by indomethacin as well as actinomycin D, while cycloheximide surprisingly potentiated PGE2 synthesis in response to both IL-1β and TNFα. IL-1α alone was ineffective in stimulating a significant release of PGE2 at concentrations as high as 10 nM. However, it also showed a marked synergistic interaction with TNFα in stimulating PGE2 release. © 1990.

引用

页码：289 / 292

页数：4

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