EVIDENCE FOR INVOLVEMENT OF PROTEIN-KINASE-C IN EXPRESSION OF INTRACELLULAR ADHESION MOLECULE-1 (ICAM-1) BY HUMAN VASCULAR ENDOTHELIAL-CELLS

被引：17

作者：

SUNG, CP ^{[1
]}

ARLETH, AJ ^{[1
]}

NAMBI, P ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT RENAL PHARMACOL,KING OF PRUSSIA,PA

来源：

PHARMACOLOGY | 1994年 / 48卷 / 03期

关键词：

ADHESION MOLECULE; ICAM-1; PKC; ENDOTHELIAL CELLS;

D O I：

10.1159/000139173

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Potent activators of protein kinase C (PKC), such as phorbol dibutyrate and octylindolactam V, stimulated expression of intercellular adhesion molecule 1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) in a concentration-dependent manner. Expression of PKC activator-induced ICAM-1 in HUVEC was inhibited by the PKC inhibitor, H-7. Furthermore, cytokine (TNF alpha, LPS)-induced ICAM-1 expression was inhibited by the potent PKC inhibitor, H-7, and not by the cAMP-dependent protein kinase (PKA) specific inhibitor, H-89. These data suggest that PKC is involved in cytokine- and inflammatory agent-induced upregulation of ICAM-1 expression in HUVEC.

引用

页码：143 / 146

页数：4

共 9 条

[1]

ASHENDEL CL, 1983, CANCER RES, V43, P4333

[2]

CASTAGNA M, 1982, J BIOL CHEM, V257, P7847

[3]

CHIJIWA T, 1990, J BIOL CHEM, V265, P5267

[4] STIMULATION OF DNA-SYNTHESIS IN MURINE FIBROBLASTS BY THE TUMOR PROMOTER TELEOCIDIN - RELATIONSHIP TO PHORBOL ESTERS AND VASOPRESSIN [J].

COLLINS, M ;

ROZENGURT, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 104 (04) :1159-1166

[5] LYMPHOCYTE FUNCTION ASSOCIATED ANTIGEN-1 (LFA-1) INTERACTION WITH INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) IS ONE OF AT LEAST 3 MECHANISMS FOR LYMPHOCYTE ADHESION TO CULTURED ENDOTHELIAL-CELLS [J].

DUSTIN, ML ;

SPRINGER, TA .

JOURNAL OF CELL BIOLOGY, 1988, 107 (01) :321-331

[6] NEW CLASSES OF TUMOR PROMOTERS - TELEOCIDIN, APLYSIATOXIN, AND PALYTOXIN [J].

FUJIKI, H ;

SUGIMURA, T .

ADVANCES IN CANCER RESEARCH, 1987, 49 :223-264

[7] ISOQUINOLINESULFONAMIDES, NOVEL AND POTENT INHIBITORS OF CYCLIC-NUCLEOTIDE DEPENDENT PROTEIN-KINASE AND PROTEIN KINASE-C [J].

HIDAKA, H ;

INAGAKI, M ;

KAWAMOTO, S ;

SASAKI, Y .

BIOCHEMISTRY, 1984, 23 (21) :5036-5041

[8] PHORBOL DIESTER RECEPTOR CO-PURIFIES WITH PROTEIN KINASE-C [J].

NIEDEL, JE ;

KUHN, LJ ;

VANDENBARK, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :36-40

[9]

ROTHLEIN R, 1986, J IMMUNOL, V137, P1270

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