A SELECTIVE LAMBDA-PHAGE CLONING VECTOR WITH AUTOMATIC EXCISION OF THE INSERT IN A PLASMID

被引：13

作者：

MARUYAMA, IN ^{[1
]}

BRENNER, S ^{[1
]}

机构：

[1] MRC,MOLEC GENET UNIT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

GENE | 1992年 / 120卷 / 02期

关键词：

SPI SELECTION; CAENORHABDITIS-ELEGANS; CDNA LIBRARY; BACTERIOPHAGE P1; CRE-LOX SITE-SPECIFIC RECOMBINATION; PHAGEMID;

D O I：

10.1016/0378-1119(92)90086-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A bacteriophage lambda cloning vehicle has been constructed for the generation of cDNA libraries. The vector has the following properties. (1) It has a unique BamHI site engineered into the lambda gam gene. Segments of DNA can be cloned into this site and clones with an insert can be selected by their ability to grow on an Escherichia coli host lysogenic for phage P2 (Spi-phenotype). (2) When the recombinant phage infects a Cre-producing E. coli strain, a site-specific recombination event results in the excision of a plasmid replicon with the cloned insert. (3) Single-stranded DNAs can be recovered by growing helper M13 phages on bacteria harboring such plasmids. The vector, lambdaMGU2, has been used to construct a nematode (Caenorhabditis elegans) cDNA library.

引用

页码：135 / 141

页数：7

共 36 条

[1] STUDIES ON THE PROPERTIES OF P1 SITE-SPECIFIC RECOMBINATION - EVIDENCE FOR TOPOLOGICALLY UNLINKED PRODUCTS FOLLOWING RECOMBINATION [J].