ACYL-THIOESTERASE ACTIVITY IN DEVELOPING SEEDS OF COCOA

被引：10

作者：

GRIFFITHS, G

WALSH, MC

HARWOOD, JL

机构：

[1] Department of Biochemistry, University of Wales College of Cardiff, Cardiff

来源：

PHYTOCHEMISTRY | 1993年 / 32卷 / 06期

关键词：

THEOBROMA-CACAO; STERCULIACEAE; COCOA; LIPID SYNTHESIS; SATURATED FATTY ACIDS; ACYL-THIOESTERASES;

D O I：

10.1016/0031-9422(93)85146-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acyl-thioesterase activity has been examined in plastid preparations isolated from two development stages in cocoa reflecting low (105 days post anthesis, dpa) and high (130 dpa) stearate (18:0) production. When supplied with an equimolar mixture of [C-14]acyl-CoAs, both preparations showed a slight preference for palmitoyl-CoA over stearoyl-CoA and oleoyl-CoA. By contrast, the order of preference for mixed [C-14]acyl-ACPs was oleate > stearate > > palmitate. Again there was no change in selectivity with age of the tissue. The results indicate that during maturation the increased accumulation of stearate in cocoa butter does not reflect the induction of a new thioesterase with increased selectivity towards stearoyl-ACP.

引用

页码：1403 / 1405

页数：3

共 17 条

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[2]

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[3]

Gunstone FD, 1986, LIPID HDB

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